Overview

  • Product name
    Anti-Human Papillomavirus 16 (E7) antibody [TVG 701Y]
    See all Human Papillomavirus 16 (E7) primary antibodies
  • Description
    Mouse monoclonal [TVG 701Y] to Human Papillomavirus 16 (E7)
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, Flow Cyt, IHC-Frmore details
  • Immunogen

    Full length protein (HPV 16 E7) synthesised from S. pombe.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A/G purified
  • Clonality
    Monoclonal
  • Clone number
    TVG 701Y
  • Myeloma
    NS0/1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription
  • Cellular localization
    Nuclear
  • Alternative names
    • E7 antibody
    • HPV16 E7 antibody
    • Protein E7 antibody
    • Transforming protein antibody

References

This product has been referenced in:
  • Yang J  et al. Notch signaling is important for epithelial-mesenchymal transition induced by low concentrations of doxorubicin in osteosarcoma cell lines. Oncol Lett 13:2260-2268 (2017). Read more (PubMed: 28454389) »
  • Kannan A  et al. Genetic Mutation and Exosome Signature of Human Papilloma Virus Associated Oropharyngeal Cancer. Sci Rep 7:46102 (2017). Read more (PubMed: 28383029) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer

Thank you for contacting us and for your patience.

The supplying lab let us know the following:

We do not have a protocol sincewe are provided our antibodies by outside scientists and we tend to see only the information that is provided to us.



I am very sorry to not be able to help you better.
Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your enquiry and your interest in our products.

These two monoclonal antibodies recognize the same protein (Human Papillomavirus 16 (E7)) but are from different clones.

https://www.abcam.com/ab20191:

https://www.abcam.com/ab30731:

As you can see on the datasheets, the concentration, the tested application and the storage buffer are different.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Answer

Thank you for your enquiry. I am pleased to help answer your questions.

1. I have contacted the originator and regrettably they are not able to provide any further information on the immunogen on this occasion.We aim to provide as much information as possible to customers and I am sorry I am unable to give you more details on this occasion.

2.The current lots we have in stock are as follows:

GR62142 is 1.3 mg/ml
GR74578 is 1.35 mg/ml

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question
Answer

Thank you for your call today and for your questions about ab20191.

I have done a search on google scholar for the clone TVG 701Y, and there are several publications listed:

http://scholar.google.com/scholar?q=TVG+701Y&hl=en&as_sdt=1%2C22&as_sdtp=on

I have downloaded the pdf files for the first two listings and attached them to this email.

I hope this information will be helpful in determining whether this antibody will work with your studies. If there is anything else that we can do for you, please don't hesitate to ask. Have a great weekend!

Read More

Answer

Thank you for contacting Abcam.   I have contacted the lab about ab20191, which is a Human Papillomavirus 16 (E7) antibody [TVG701Y] and can be used in flow cytometry and western blot. However the lab has told me that we do not know what the immunogen sequence is for this antibody.   This paper might be of help to you though:   http://www.ncbi.nlm.nih.gov/pubmed?term=8380917    If there is anything else I can help you with, please let me know.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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