Overview

  • Product name
    Human Anti-Parainfluenza virus 1,2,3 IgG ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 8 6.8%
    Pos.Serum 8 1.6%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 3 9.8%
  • Sample type
    Serum, Heparin Plasma, Citrate Plasma
  • Assay type
    Indirect
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Parainfluenza virus 1, 2, 3 IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Parainfluenza virus 1, 2, 3 in Human serum and plasma.

     

    A 96-well plate has been precoated with Parainfluenza virus 1, 2, 3 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Parainfluenza virus 1, 2, 3-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Parainfluenza virus 1, 2, 3 IgG sample captured in plate.

  • Tested applications
    Suitable for: Indirect ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References

ab108758 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question

I recently bought one ab108758 ELISA kit and have recently purchased. It is the first time that I am using your company and this kit.

I have some questions regarding the controls and the quantification of results.

Firstly, can you please tell me the concentration of antibody in the positive control and inform me of the significance of the cut off control- why are they needed and what exactly is in the bottle. Also, with regards to the negative control, I assume it contains no antibody, just some serum, is this correct?

Secondly, in an experiment, my blank well came up higher than is recommended in your protocol booklet, are there any other reasons apart from poor washing/well contamination that could account for this? And, is it necessary for me to repeat the experiment again if my blank and negative controls are higher than your recommendations?

Also, with regards to quantification of results, if some of my samples are higher than the positive control what does this mean? -Should my results, using my own samples, be between the positive control and the cut-off control in order to be classed as positive.

Is there a way to convert abcam units into concentration? Is there a possibility to purchase known concentrations of a IgG to be used as a standard curve that can be used to extrapolate concentrations?

Lastly, in your ELISA protocol there is no blocking step to prevent non-specific binding. Does your protocol already account for this somewhere that I have missed or is it not necessary for this ELISA? Do you recommend the addition of a blocking step?

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Answer

1.)  ab108758 is a qualitative ELISA, the concentration of the parainfluenza specific antibodies in the positive control is not determined. In order to calculate the concentration in international units the existence of an international standard is necessary. This kind of standard does not exist. It is only possible to calculate the concentration in arbitrary units (NTU) by deviding the OD of the positive control by the mean OD of the cut-off control.
The negative and positive controls are needed in order to check if the test run was principally correct. If these controls are not found inside the validation criteria, there may be a problem with the processing or with the kit. The cut-off control is the most important control since it distinguishes between positive and negative samples.
All controls are a mixture of a buffer system and human serum or plasma.
2.) I can confirm it is necessary to repeat the run. How is the assay performed manually or with an automatic system? For further support it would be necessary to get the complete results of the run.
3.) The positive control is only important for the validation of the run. It is not needed for the result calculation. Positive samples can be higher or lower than the positive control. The definition is that samples with > 11 NTU are positive.
4.) It is not possible to convert abcam units into a concentration. 
5.) A blocking step is not necessary. The microplates are produced in a way that non-specific binding is eliminated.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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