Overview

  • Product name

    Human PARK7 Matched Antibody Pair Kit
    See all PARK7/DJ1 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    312.5 pg/ml - 20000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human PARK7.


    Both capture and detector antibodies are rabbit monoclonal antibodies.


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab215535) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human PARK7 Capture Antibody 1 x 100µg 1 x 20µg
    Human PARK7 Detector Antibody 1 x 25µg 1 x 5µg
    Human PARK7 Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone.
  • Tissue specificity

    Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain. Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.
  • Involvement in disease

    Defects in PARK7 are the cause of Parkinson disease type 7 (PARK7) [MIM:606324]. A neurodegenerative disorder characterized by resting tremor, postural tremor, bradykinesia, muscular rigidity, anxiety and psychotic episodes. PARK7 has onset before 40 years, slow progression and initial good response to levodopa. Some patients may show traits reminiscent of amyotrophic lateral sclerosis-parkinsonism/dementia complex (Guam disease).
  • Sequence similarities

    Belongs to the peptidase C56 family.
  • Post-translational
    modifications

    Sumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.
    Cys-106 is easily oxidized to sulfinic acid.
    Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.
  • Cellular localization

    Cytoplasm. Nucleus. Mitochondrion. Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients.
  • Information by UniProt
  • Alternative names

    • CAP1
    • DJ-1
    • DJ1
    • DJ1 protein
    • Epididymis secretory sperm binding protein Li 67p
    • FLJ27376
    • FLJ34360
    • FLJ92274
    • HEL S 67p
    • Oncogene DJ1
    • OTTHUMP00000001348
    • OTTHUMP00000001349
    • OTTHUMP00000001350
    • OTTHUMP00000001351
    • PARK7
    • PARK7_HUMAN
    • Parkinson disease (autosomal recessive, early onset) 7
    • Parkinson disease protein 7
    • Parkinson protein 7
    • Protein DJ-1
    • SP22
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab218797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab218797 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab218797.
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