Key features and details
- One-wash 90 minute protocol
- Sensitivity: 133 pg/ml
- Range: 300 pg/ml - 5000 pg/ml
- Sample type: Adherent cells, Cell culture extracts, Cell Lysate, Suspension cells, Tissue Extracts, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman PBK ELISA Kit
See all PDZ Binding Kinase kits
Intra-assay Sample n Mean SD CV% Jurkat cells 5 2% Inter-assay Sample n Mean SD CV% Jurkat cells 3 14%
Sample typeCell culture extracts, Adherent cells, Suspension cells, Tissue Extracts, Cell Lysate, Tissue Homogenate
Assay typeSandwich (quantitative)
Range300 pg/ml - 5000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture media 103 97% - 109% Extraction Buffer 100 99% - 100%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human PBK (PDZ Binding Kinase) ELISA kit (ab184866) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PBK protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human PDZ Binding Kinase with 133 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
PDZ-binding kinase (also known PBK or Lymphocyte-activated killer T-cell-originated protein kinase) is a Serine/Threonine protein kinase in the MAP kinase kinase subfamily. PBK phosphorylates MAP kinase p38. PBK itself is phosphorylated and appears to be active only during the mitotic phase of the cell cycle. When phosphorylated PBK may interact with TP53. PBK may play a role in the activation of lymphoid cells and is found to expressed in many highly proliferative cell lines.
The standard is affinity purified.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human PDZ Binding Kinase Capture Antibody 1 x 600µl 10X Human PDZ Binding Kinase Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human PDZ Binding Kinase Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
- Cancer/testis antigen 84
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of PBK protein were interpolated from the PBK standard curve and plotted against the extract load.
The concentrations of PBK protein were interpolated from the PBK standard curve and corrected for sample dilution relative to extract load of 35.7 µg/mL. The mean PBK concentration was determined to be 6.95 ng/mL in A431 extract at 35.7 µg/mL.
Interpolated values of PBK are plotted for the indicated cell lines based on a extract load of 250 µg/mL.
To learn more about the advantages of recombinant antibodies see here.
ab184866 has not yet been referenced specifically in any publications.