Overview

  • Product name

    Human PCSK9 ELISA Kit
    See all PCSK9 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human serum 5 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Human serum 3 4.6%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    68 pg/ml
  • Range

    0.5 ng/ml - 32 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106 103% - 109%
    Serum 105 104% - 107%
    Cell culture extracts 92 91% - 94%
    Heparin Plasma 111 107% - 115%
    EDTA Plasma 96 93% - 100%
    Citrate Plasma 104 102% - 106%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow, Cynomolgus monkey, Macaque monkey
  • Product overview

    Abcam’s PCSK9 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PCSK9 protein in human serum, plasmas, cell culture supernatants, and cell extracts.


     The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material.  TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Senssitivity:


    Samples diluted in Sample Diluent NS: 83 pg/mL


    Samples diluted in 1X Cell Extraction Buffer PTR: 68 pg/mL


     

  • Notes

    Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial player in the regulation of plasma cholesterol homeostasis. Degradation of low-density lipid receptor family members (LDLR, VLDLR, APOER, and APOER2) is promoted in intracellular acidic compartments following binding by PCSK9.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab209884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between human PCSK9 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold increase in sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of PCSK9 were measured in duplicates, interpolated from the PCSK9 standard curves and corrected for sample dilution. Undiluted samples are as follows:  serum 10%, plasma (citrate) 10%, and plasma (heparin) 10%.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean PCSK9 concentration was determined to be 207 ng/mL in serum, 117 ng/mL in plasma (citrate), 166 ng/mL in plasma (EDTA), and 292 ng/mL in plasma (heparin).

  • The concentrations of PCSK9 were measured in duplicate, interpolated from the PCSK9 standard curve, and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 50%, HepG2 cell extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean PCSK9 concentration was determined to be 7.3 ng/mL in HepG2 cell culture supernatant and 15 ng/mL in HepG2 cell extract.

     

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean PCSK9 concentration was determined to be 208 ng/mL with a range of 125 - 317 ng/mL.

Protocols

References

This product has been referenced in:

  • Tóth Š  et al. Elevated Circulating PCSK9 Concentrations Predict Subclinical Atherosclerotic Changes in Low Risk Obese and Non-Obese Patients. Cardiol Ther 6:281-289 (2017). Read more (PubMed: 28623549) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

The antibodies were raised against the C terminal fragment, but the epitope has not been mapped. So most likely it will not recognize the furin cleaved N terminal fragment, but will recognize the  C terminal fragment.

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