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  1. Link

    human-pcsk9-matched-antibody-pair-kit-ab215081.pdf

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Signal Transduction Metabolism Amino Acids
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Recombinant

Human PCSK9 Matched Antibody Pair Kit (ab215081)

  • Datasheet
  • SDS
  • Protocol Booklet
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Human PCSK9 standard curve.
  • Sandwich ELISA - Human PCSK9 Matched Antibody Pair Kit (ab215081)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Human
  • Range: 250 pg/ml - 16000 pg/ml

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Overview

  • Product name

    Human PCSK9 Matched Antibody Pair Kit
    See all PCSK9 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    37.07 pg/ml
  • Range

    250 pg/ml - 16000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human PCSK9 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human PCSK9.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab209884. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: IA, ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 5 x 96 tests
    Human PCSK9 Capture Antibody 1 x 50µg
    Human PCSK9 Detector Antibody 1 x 12.5µg
    Human PCSK9 Lyophilized Protein 1 vial
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Serine protease
    • Proprotein Convertases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Neuroscience
    • Processes
  • Function

    May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
  • Tissue specificity

    Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
  • Involvement in disease

    Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
  • Sequence similarities

    Belongs to the peptidase S8 family.
    Contains 1 peptidase S8 domain.
  • Post-translational
    modifications

    The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q8NBP7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Convertase subtilisin/kexin type 9 preproprotein
    • FH3
    • HCHOLA3
    • Hypercholesterolemia autosomal dominant 3
    • LDLCQ1
    • NARC 1
    • NARC-1
    • NARC1
    • Neural apoptosis regulated convertase 1
    • Neural apoptosis-regulated convertase 1
    • PC 9
    • PC9
    • PCSK 9
    • PCSK9
    • PCSK9_HUMAN
    • Proprotein convertase 9
    • Proprotein convertase PC9
    • Proprotein convertase subtilisin/kexin type 9
    • PSEC0052
    • Subtilisin/kexin like protease PC9
    • Subtilisin/kexin-like protease PC9
    see all
  • Database links

    • Entrez Gene: 255738 Human
    • Omim: 607786 Human
    • SwissProt: Q8NBP7 Human
    • Unigene: 18844 Human

    Associated products

    • Alternative Versions

      • Human PCSK9 Antibody Pair - BSA and Azide free (ab241810)
    • Related Products

      • ELISA Accessory Pack (ab210905)
    • SimpleStep ELISA kits

      • Human PCSK9 ELISA Kit (ab209884)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab215081 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IA
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.
    Notes
    IA
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.

    Images

    • Human PCSK9 standard curve.
      Human PCSK9 standard curve.

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Human PCSK9 Matched Antibody Pair Kit (ab215081)
      Sandwich ELISA - Human PCSK9 Matched Antibody Pair Kit (ab215081)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab215081? Please let us know so that we can cite the reference in this datasheet.

    ab215081 has not yet been referenced specifically in any publications.

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