• Product name
    Human PDGF AA ELISA Kit
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 40 pg/ml
  • Range
    40 pg/ml - 30000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 92.54 82% - 102%
    Serum 76.71 70% - 83%
    Plasma 78.06 72% - 85%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PDGF AA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF AA in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human PDGF AA coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF AA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF AA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Human PDGF AA (lyophilized) 2 vials
    PDGF AA Microplate (12 x 8 wells) 1 unit
    Recombinant Human PDGF AA Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Domain
    The long form contains a basic insert which acts as a cell retention signal.
  • Cellular localization
    Secreted. Released by platelets upon wounding.
  • Information by UniProt
  • Alternative names
    • PDGF 1
    • PDGF A
    • PDGF A chain
    • PDGF subunit A
    • PDGF-1
    • PDGF1
    • PDGFA
    • Platelet derived growth factor A chain
    • Platelet derived growth factor alpha chain
    • Platelet derived growth factor alpha polypeptide
    • Platelet derived growth factor subunit A
    • Platelet-derived growth factor A chain
    • Platelet-derived growth factor alpha polypeptide
    • Platelet-derived growth factor subunit A
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab100622.



This product has been referenced in:
  • Wood MD  et al. Neurofibromin knockdown in glioma cell lines is associated with changes in cytokine and chemokine secretion in vitro. Sci Rep 8:5805 (2018). Read more (PubMed: 29643433) »

See 1 Publication for this product

Customer reviews and Q&As

Thank for your enquiry and interest.

I would like to confirm the this kit contains 2 vials of Standards: recombinant human PDGF-AA

However, currently we also have we have 4 products PDGF...

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Thank you for your enquiry. From the search results you have provided, I can confirm that the following PDGF ELISA kits are tested on cell culture samples and will be suitable for this sample type. I can suggest to select one that has been test...

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