Key features and details
- Sensitivity: 10 pg/ml
- Range: 10.24 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman PDGF AB ELISA Kit
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 10 pg/ml
Range10.24 pg/ml - 2500 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 74.29 68% - 78% Serum 73.96 67% - 78% Plasma 74.85 67% - 79%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s PDGF AB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF AB in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human PDGF AB coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF AB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF AB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human PDGF AB (lyophilized) 2 vials PDGF AB Microplate (12 x 8 wells) 1 unit Recombinant Human PDGF AB Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevancePlatelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains joined by disulphide bonds, termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain.
- PDGF A chain
- PDGF B chain
ab100623 has not yet been referenced specifically in any publications.