Overview

  • Product name

    Human PDGF BB ELISA Kit
    See all PDGF BB kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 1 pg/ml
  • Range

    0.549 pg/ml - 400 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.43 84% - 104%
    Serum 93.57 82% - 103%
    Plasma 91.99 83% - 104%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s PDGF BB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF BB in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human PDGF BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


     Get results in 90 minutes with Human PDGF BB ELISA Kit (ab184860) from our SimpleStep ELISA® range.

  • Notes

    Note: Optimization may be required with urine samples, and very low level are detectable in serum

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    800X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human PDGF BB 2 vials
    PDGF BB Microplate (12 x 8 wells) 1 unit
    Recombinant Human PDGF BB Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
  • Tissue specificity

    Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
  • Involvement in disease

    Note=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization

    Secreted. Released by platelets upon wounding.
  • Information by UniProt
  • Alternative names

    • Becaplermin
    • c sis
    • FLJ12858
    • Oncogene SIS
    • PDG B chain
    • PDGB
    • PDGF 2
    • PDGF B
    • PDGF B chain
    • PDGF subunit B
    • PDGF-2
    • PDGF2
    • Pdgfb
    • PDGFB/COL1A1 fusion gene
    • PDGFB_HUMAN
    • Platelet derived growth factor 2
    • Platelet derived growth factor B chain
    • Platelet derived growth factor beta polypeptide
    • Platelet derived growth factor beta polypeptide (oncogene SIS)
    • Platelet derived growth factor subunit B
    • Platelet-derived growth factor B chain
    • Platelet-derived growth factor beta polypeptide
    • Platelet-derived growth factor subunit B
    • Proto oncogene c Sis
    • Proto-oncogene c-Sis
    • Simian sarcoma viral (v sis) oncogene homolog
    • SIS
    • SSV
    • V sis platelet derived growth factor beta polypeptide
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100624.

  • Representative Standard Curve using ab100624.

Protocols

References

This product has been referenced in:

  • Lin TW  et al. Establishing Liposome-Immobilized Dexamethasone-Releasing PDMS Membrane for the Cultivation of Retinal Pigment Epithelial Cells and Suppression of Neovascularization. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30634448) »
  • Zhang H  et al. MiR-148b-3p inhibits renal carcinoma cell growth and pro-angiogenic phenotype of endothelial cell potentially by modulating FGF2. Biomed Pharmacother 107:359-367 (2018). Read more (PubMed: 30099339) »
See all 6 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Thank you very much for your reply. My colleague is out of the office but I'm happy to help you with this issue.

Your credit note ID is *** I am sorry that thiskit did not perform as expected. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department at mailto:us.credits@abcam.com so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Also, could you tell me what kind of cells were used with the kit? We would just like to collect that information so we can monitor the quality of this kit. Thanks so much for your assistance.

Please let me know if you have any questions or if there is anything else that we can do for you.

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Answer

Thank you for your reply.

We do have 10 kDa spin colums for ultrafiltration available as ab93349 and concentration kits available, as well. I'm not exactly sure how you would like to concentrate your samples, but here is the link to our current kit offering:

https://www.abcam.com/index.html?pageconfig=resource&rid=13489

I hope this information helps. Please contact us with any other questions.

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Answer

Thank you for contacting Abcam.

Please find the answers to your questions below

1 - Will FCS interfer with the assay signal?
No. However, since serum-containing media does contain cytokines, I would recommend that the you also run an uncultured media blank sample to assess the media’s baseline response.
2 - Can you use froxen supernatent or does it have to be fresh?
Frozen cell culture supernatant samples are fine.
3 - Signal was weak after 2.5hrs incubation at room tem. Will overnight at 4C help?
Performing the standard/sample incubation step overnight is the most effective way increase the overall plate signal. There are also other steps that can tried, which I have listed below.

Increase amount of biotinylated antibody (by 1.5 fold or so – too much may increase background)
Increase amount of HRP-streptavidin (by about 1.5 fold or so – too much may increase background)
Concentrate sample in a non-denaturing method (for example, using a chilled spin column)


Please note: it’s best to try just one of these modifications at a time. Implementing too many of these changes at once may cause high background.

Please let me know if you have any questions or comments.

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Question
Answer

Thank you once again for your enquiries.
I can confirm this component has now been added as a separate item to the catalog.

Please use this product code when placing your order:
ab135400 HRP-Streptavidin Concentrate 800x concentrated HRP-conjugated streptavidin

https://www.abcam.com/index.html?datasheet=135400 (or use the following: https://www.abcam.com/index.html?datasheet=135400).

The price and further information are on the online datasheet:
UK price: 200 ul £65.00
Shippping UK: £16.00

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your telephone call yesterday afternoon.

I have contacted my colleagues who have informed me that we have some website and administration changes taking place this week, so regrettably I am not able to do this for you today. However, I should be able to arrange this for you later in the week or early next week and let you know the product code and cost.

Could you confirm you would still want to go ahead and order this?

I appreciate your patience and look forward to hearing from you.

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Answer

Thank you for contacting Abcam.

Please find attached the Certificate of Compliances for ab100624 and ab100626.

If there is anything else I can help you with, please do not hesitate to contact me.

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Question
Answer

Thank you for contacting Abcam. Attached, please find the Certificates of Compliance you requested. I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

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Answer

Thank you for your enquiry. From the search results you have provided, I can confirm that the following PDGF ELISA kits are tested on cell culture samples and will be suitable for this sample type. I can suggest to select one that has been tested in the species the customer will be testing. ab100622 PDGF AA Human ELISA Kit ab100623 PDGF AB Human ELISA Kit ab100624 PDGF BB Human ELISA Kit ab100625 PDGF BB Human ELISA Kit The A and B refer to the subunits and isoforms of the protein. Reviewing the EST expression profiles on the Unigene database, both the A and B subunits are expressed in lung, but A is expressed more than B. However, I can suggest the customer performs a further literature search to confirm this and to obtain more information. Please note that ab100626 included in the search results you provided detects the PDGF beta receptor, not the PDGF itself. I hope this information will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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