• Product name
    Human PDGF BB ELISA Kit
    See all PDGF BB kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    0.549 pg/ml - 400 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.43 84% - 104%
    Serum 93.57 82% - 103%
    Plasma 91.99 83% - 104%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PDGF BB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF BB in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human PDGF BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

     Get results in 90 minutes with Human PDGF BB ELISA Kit (ab184860) from our SimpleStep ELISA® range.

  • Notes

    Note: Optimization may be required with urine samples, and very low level are detectable in serum

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    800X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human PDGF BB 2 vials
    PDGF BB Microplate (12 x 8 wells) 1 unit
    Recombinant Human PDGF BB Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
  • Tissue specificity
    Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
  • Involvement in disease
    Note=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization
    Secreted. Released by platelets upon wounding.
  • Information by UniProt
  • Alternative names
    • Becaplermin
    • c sis
    • FLJ12858
    • Oncogene SIS
    • PDG B chain
    • PDGB
    • PDGF 2
    • PDGF B
    • PDGF B chain
    • PDGF subunit B
    • PDGF-2
    • PDGF2
    • Pdgfb
    • PDGFB/COL1A1 fusion gene
    • Platelet derived growth factor 2
    • Platelet derived growth factor B chain
    • Platelet derived growth factor beta polypeptide
    • Platelet derived growth factor beta polypeptide (oncogene SIS)
    • Platelet derived growth factor subunit B
    • Platelet-derived growth factor B chain
    • Platelet-derived growth factor beta polypeptide
    • Platelet-derived growth factor subunit B
    • Proto oncogene c Sis
    • Proto-oncogene c-Sis
    • Simian sarcoma viral (v sis) oncogene homolog
    • SIS
    • SSV
    • V sis platelet derived growth factor beta polypeptide
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab100624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab100624.

  • Representative Standard Curve using ab100624.



This product has been referenced in:
  • Steinecker-Frohnwieser B  et al. Pharmacological treatment with diacerein combined with mechanical stimulation affects the expression of growth factors in human chondrocytes. Biochem Biophys Rep 11:154-160 (2017). Sandwich ELISA ; Human . Read more (PubMed: 28955780) »
  • Tanaka K  et al. miR-27 is associated with chemoresistance in esophageal cancer through transformation of normal fibroblasts to cancer-associated fibroblasts. Carcinogenesis 36:894-903 (2015). ELISA ; Human . Read more (PubMed: 26026166) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you very much for your reply. My colleague is out of the office but I'm happy to help you with this issue.

Your credit note ID is *** I am sorry that thiskit did not perform as expected. If payment has already been made on the original...

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We do have 10 kDa spin colums for ultrafiltration available as ab93349 and concentration kits available, as well. I'm not exactly sure how you would like to concentrate your samples, but here is the link to our curre...

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Thank you for contacting Abcam.

Please find the answers to your questions below

1 - Will FCS interfer with the assay signal?
No. However, since serum-containing media does contain cytokines, I would recommend that the you also ...

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Thank you once again for your enquiries.
I can confirm this component has now been added as a separate item to the catalog.

Please use this product code when placing your order:
ab135400 HRP-Streptavidin Concentrate 800x concentrat...

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I have contacted my colleagues who have informed me that we have some website and administration changes taking place this week, so regrettably I am not able to do this for you today. Ho...

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Please find attached the Certificate of Compliances for ab100624 and ab100626.

If there is anything else I can help you with, please do not hesitate to contact me.

Thank you for contacting Abcam. Attached, please find the Certificates of Compliance you requested. I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

Thank you for your enquiry. From the search results you have provided, I can confirm that the following PDGF ELISA kits are tested on cell culture samples and will be suitable for this sample type. I can suggest to select one that has been test...

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