Overview

  • Product name
    Human PDGF BB ELISA Kit
    See all PDGF BB kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Human Serum 8 3.3%
    Inter-assay
    Sample n Mean SD CV%
    Human Serum 3 5.3%
  • Sample type
    Serum, Plasma, Cell culture media
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    2.1 pg/ml
  • Range
    2.06 pg/ml - 1500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 95 85% - 100%
    Cell culture media 104 101% - 106%
    Heparin Plasma 104 92% - 114%
    EDTA Plasma 99 91% - 107%
    Citrate Plasma 109 100% - 114%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PDGF BB in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PDGF-BB protein in human serum, plasma, and cell culture supernatant.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm. 

  • Notes

    Platelet derived growth factor B chain plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. As a homodimer it is a potent mitogen for cells of mesenchymal origin and is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. PDGF-BB is essential for normal blood vessel development, for normal development of kidney glomeruli and in wound healing. Signaling is modulated by the formation of heterodimers with PDGF A chain to yield PDGF-AB.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X PDGF BB Capture Antibody 1 x 600µl
    10X PDGF BB Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    ab221824 - Antibody Diluent 4BI 1 x 6ml
    PDGF BB Human Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas
  • Function
    Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
  • Tissue specificity
    Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
  • Involvement in disease
    Note=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Cellular localization
    Secreted. Released by platelets upon wounding.
  • Information by UniProt
  • Alternative names
    • Becaplermin
    • c sis
    • FLJ12858
    • Oncogene SIS
    • PDG B chain
    • PDGB
    • PDGF 2
    • PDGF B
    • PDGF B chain
    • PDGF subunit B
    • PDGF-2
    • PDGF2
    • Pdgfb
    • PDGFB/COL1A1 fusion gene
    • PDGFB_HUMAN
    • Platelet derived growth factor 2
    • Platelet derived growth factor B chain
    • Platelet derived growth factor beta polypeptide
    • Platelet derived growth factor beta polypeptide (oncogene SIS)
    • Platelet derived growth factor subunit B
    • Platelet-derived growth factor B chain
    • Platelet-derived growth factor beta polypeptide
    • Platelet-derived growth factor subunit B
    • Proto oncogene c Sis
    • Proto-oncogene c-Sis
    • Simian sarcoma viral (v sis) oncogene homolog
    • SIS
    • SSV
    • V sis platelet derived growth factor beta polypeptide
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab184860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of PDGF-BB standard curve for ab184860

  • Titration of Human serum with ab184860 within the working range of the assay. Background subtracted data from duplicate measurements are plotted.

  • Observed PDGF-BB levels in pooled donor normal Human plasma and normal Human platelet-poor plasma (PP-Plasma). Mean Human PDGF-BB values fall within expected normal reference ranges (WHO).

  • ab184860 was validated for assay specificity against PDGF-A protein. Background-subtracted data values (mean +/- SD) are graphed. In addition, PDGF-A was prepared at 15 ng/mL and assayed for cross reactivity. No cross reactivity or interference was observed.

  • Observed PDGF-BB levels in individual donor normal Human serum (n=10). Mean Human PDGF-BB is 145 pg/mL and values fall within expected normal reference ranges (WHO).

  • Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 µg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed for endogenous PDGF-BB levels.

Protocols

References

ab184860 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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