• Product name

    Human PDH E1 alpha protein ELISA Kit (PDHA1)
    See all Pyruvate Dehydrogenase E1-alpha subunit kits
  • Detection method

  • Precision

    Sample n Mean SD CV%
    HeLa extract 2 7.7%
    Sample n Mean SD CV%
    HeLa extract 5 3.8%
  • Sample type

    Tissue Extracts, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    12 ng/ml
  • Range

    15.62 ng/ml - 1000 ng/ml
  • Recovery

    135 %

    Sample specific recovery
    Sample type Average % Range
    Serum 144 129% - 152%
    Cell culture media 135 134% - 137%
    Fetal Bovine Serum 135 129% - 130%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s PDH E1 alpha protein in vitro Human SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of PDH E1 alpha protein in Human cell and tissue extracts.

    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm.Optionally,instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    The pyruvate dehydrogenase complex performs the decarboxylation of pyruvate into acetyl CoA, a critical function in metabolism, linking glycolysis and oxidative phosphorylation in mitochondria. The enzyme is composed of multiple copies of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide transacetylase (E2) and dihydrolipoamide dehydrogenase (E3). The E1 enzyme is a tetramer of two alpha (PDHA1) and two beta (PDHB) subunits and is present in 30 copies in the PDH complex.


    The activity of PDH is regulated by reversible phosphorylation of three serine residues on the PDHA1 subunit at phospho S232, phospho S293, and phospho S300. ELISA assays to measure total PDHA1, phospho 232, phospho S293, and phospho S300 are available from Abcam (ab115342, ab115343, ab115344, ab115345). The phosphorylation of these sites is catalyzed by PDH kinases (PDK). There are four known PDK isoforms, distributed differently in tissues. Their expressions are regulated differently by factors such as starvation, hypoxia and utilization of glucose and fatty acids in various tissues. Dephosporylation, to restore the activity of PDH, is catalyzed by PDH phosphatases (PDP). There are two known isoforms of PDP; PDP1 is present in high levels in skeletal muscle and PDP2 in liver and adipocytes. Functional PDH kinases and phosphatases are available from Abcam (ab110359, ab110354, ab110355, ab110356).

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human PDH E1a Capture Antibody 1 x 600µl
    10X Human PDH E1a Detector Antibody 1 x 600µl
    2X Cell Extraction Buffer LM 1 x 10ml
    2X Cell Extraction Buffer LM 1 x 10ml
    4X Antibody Diluent EB 1 x 6ml
    Human PDH E1 alpha Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Tissue specificity

  • Involvement in disease

    Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) [MIM:312170]. An enzymatic defect causing primary lactic acidosis in children. It is associated with a broad clinical spectrum ranging from fatal lactic acidosis in the newborn to chronic neurologic dysfunction with structural abnormalities in the central nervous system without systemic acidosis.
    Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.
  • Post-translational

    Phosphorylation at Ser-293 by PDK family kinases blocks the access to active site, and inactivates the enzyme.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Alternative names

    • mitochondrial
    • PDHA1
    • PDHCE1A
    • PDHE1-A type I
    • PHE1A
    • Pyruvate dehydrogenase (lipoamide) alpha 1
    • Pyruvate dehydrogenase complex E1 alpha polypeptide 1
    • Pyruvate dehydrogenase E1 component subunit alpha
    • Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial
    • Pyruvate dehydrogenase, alpha-1
    • somatic form
    see all
  • Database links


Our Abpromise guarantee covers the use of ab181415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.


  • Example of PDH E1 alpha (PDHA1) standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of HeLa, HepG2, Human Liver Homogenate (HLH) and MCF7 cell extract within the working range of the assay in 1X Cell Extraction Buffer LM. Background subtracted data from duplicate measurements are plotted.



This product has been referenced in:

  • Ahn E  et al. Temporal fluxomics reveals oscillations in TCA cycle flux throughout the mammalian cell cycle. Mol Syst Biol 13:953 (2017). Read more (PubMed: 29109155) »
See 1 Publication for this product

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