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    human-pedf-elisa-kit-serpinf1-ab213815.pdf

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Human PEDF ELISA Kit (SerpinF1) (ab213815)

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  • SDS
  • Protocol Booklet
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Human PEDF ELISA Kit (SerpinF1) (ab213815) Standard Curve

    Key features and details

    • Sensitivity: 20 pg/ml
    • Range: 0.78 ng/ml - 50 ng/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Overview

    • Product name

      Human PEDF ELISA Kit (SerpinF1)
      See all PEDF kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      1 16 5.62ng/ml 0.259 4.6%
      2 16 10.1ng/ml 0.444 4.4%
      3 16 25.5ng/ml 1.07 4.2%
      Inter-assay
      Sample n Mean SD CV%
      1 24 6.23ng/ml 0.467 7.5%
      2 24 12.1ng/ml 0.835 6.9%
      3 24 26.4ng/ml 1.95 7.4%
    • Sample type

      Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 20 pg/ml
    • Range

      0.78 ng/ml - 50 ng/ml
    • Assay time

      3h 30m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      The Human PEDF Enzyme-Linked Immunosorbent Assay (ELISA) kit (SerpinF1) (ab213815) is designed for the quantitative detection of Human PEDF in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). 


      The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for PEDF has been pre-coated onto 96-well plates. Standards (NSO, M1-P418) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PEDF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PEDF amount of sample captured in plate.

    • Notes

      Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, anti-tumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the anti-tumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Adhesive Plate Seal 4 units
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Human PEDF coated Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti-Human PEDF antibody 1 x 100µl
      Lyophilized recombinant Human PEDF standard 2 vials
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Black Cap 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Inhibitors
      • Neuroscience
      • Sensory System
      • Visual system
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic inhibitory factors
    • Function

      Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
    • Tissue specificity

      Retinal pigment epithelial cells and blood plasma.
    • Sequence similarities

      Belongs to the serpin family.
    • Developmental stage

      Expressed in quiescent cells.
    • Domain

      The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
    • Post-translational
      modifications

      The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
      N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • Cellular localization

      Secreted. Melanosome. Enriched in stage I melanosomes.
    • Target information above from: UniProt accession P36955 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Cell proliferation-inducing gene 35 protein
      • EPC 1
      • EPC-1
      • EPC1
      • OI12
      • OI6
      • PEDF
      • PEDF_HUMAN
      • PIG 35
      • PIG35
      • Pigment epithelium derived factor
      • Pigment epithelium-derived factor
      • Proliferation inducing protein 35
      • Serine (or cysteine) proteinase inhibitor
      • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
      • Serpin F1
      • Serpin family F member 1
      • Serpin peptidase inhibitor
      • Serpin peptidase inhibitor clade F member 1
      • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
      • SERPINF 1
      • Serpinf1
      see all
    • Database links

      • Entrez Gene: 5176 Human
      • Omim: 172860 Human
      • SwissProt: P36955 Human
      • Unigene: 532768 Human

      Images

      • Human PEDF ELISA Kit (SerpinF1) (ab213815) Standard Curve
        Human PEDF ELISA Kit (SerpinF1) (ab213815) Standard Curve

        Human PEDF ELISA Kit (SerpinF1) (ab213815) Standard Curve

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab213815? Please let us know so that we can cite the reference in this datasheet.

      ab213815 has been referenced in 3 publications.

      • Peláez-Jaramillo MJ  et al. Impact of a Formulation Containing Unusual Polyunsaturated Fatty Acids, Trace Elements, Polyphenols and Plant Sterols on Insulin Resistance and Associated Disturbances. Diabetes Ther 11:229-245 (2020). PubMed: 31691133
      • Makridakis M  et al. Multiplexed MRM-based protein quantification of putative prognostic biomarkers for chronic kidney disease progression in plasma. Sci Rep 10:4815 (2020). PubMed: 32179759
      • Al-Ani A  et al. In Vitro Maturation of Retinal Pigment Epithelium Is Essential for Maintaining High Expression of Key Functional Genes. Int J Mol Sci 21:N/A (2020). PubMed: 32842471

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