Overview

  • Product name

    Human PEDF ELISA Kit (SerpinF1)
    See all PEDF kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 5.62ng/ml 0.259 4.6%
    2 16 10.1ng/ml 0.444 4.4%
    3 16 25.5ng/ml 1.07 4.2%
    Inter-assay
    Sample n Mean SD CV%
    1 24 6.23ng/ml 0.467 7.5%
    2 24 12.1ng/ml 0.835 6.9%
    3 24 26.4ng/ml 1.95 7.4%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    0.78 ng/ml - 50 ng/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human PEDF Enzyme-Linked Immunosorbent Assay (ELISA) kit (SerpinF1) (ab213815) is designed for the quantitative detection of Human PEDF in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). 


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for PEDF has been pre-coated onto 96-well plates. Standards (NSO, M1-P418) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PEDF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PEDF amount of sample captured in plate.

  • Notes

    Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, anti-tumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the anti-tumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human PEDF coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human PEDF antibody 1 x 130µl
    Lyophilized recombinant Human PEDF standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificity

    Retinal pigment epithelial cells and blood plasma.
  • Sequence similarities

    Belongs to the serpin family.
  • Developmental stage

    Expressed in quiescent cells.
  • Domain

    The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
  • Post-translational
    modifications

    The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization

    Secreted. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt
  • Alternative names

    • Cell proliferation-inducing gene 35 protein
    • EPC 1
    • EPC-1
    • EPC1
    • OI12
    • OI6
    • PEDF
    • PEDF_HUMAN
    • PIG 35
    • PIG35
    • Pigment epithelium derived factor
    • Pigment epithelium-derived factor
    • Proliferation inducing protein 35
    • Serine (or cysteine) proteinase inhibitor
    • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
    • Serpin F1
    • Serpin family F member 1
    • Serpin peptidase inhibitor
    • Serpin peptidase inhibitor clade F member 1
    • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
    • SERPINF 1
    • Serpinf1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human PEDF ELISA Kit (SerpinF1) (ab213815) Standard Curve

Protocols

References

ab213815 has not yet been referenced specifically in any publications.

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