Product nameHuman Periodontal Disease Antibody Array (Membrane, 20 Targets)
Sample typeSerum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
Species reactivityReacts with: Human
Abcam’s Human Periodontal Disease Antibody Array (ab211064) for use with cell culture media, serum, plasma, cell and tissue lysates and other liquid samples.
Targets: IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-4, IL-6, IL-8 (CXCL8), IL-10, IL-12 p70, IFN-gamma, TGF beta 1, TNF alpha, OPG, IL-17A, MIP-1 alpha (CCL3), MMP-13, MMP-9, Osteopontin, RANK (TNFRSF11A), CRP, Osteoactivin.
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Tested applicationsSuitable for: Multiplex Protein Detectionmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 2 Membrane 4 Membrane 8 Membrane 1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Antibody Arrays 2 units 4 units 8 units Biotinylated Antibody Cocktail 2 vials 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Plastic sheets 1 unit 1 unit 1 unit
Our Abpromise guarantee covers the use of ab211064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
ab211064 has not yet been referenced specifically in any publications.