Product nameHuman Periodontal Disease Antibody Array (Membrane, 20 Targets)
Sample typeSerum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
Species reactivityReacts with: Human
Abcam’s Human Periodontal Disease Antibody Array (ab211064) for use with cell culture media, serum, plasma, cell and tissue lysates and other liquid samples.
Targets: IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-4, IL-6, IL-8 (CXCL8), IL-10, IL-12 p70, IFN-gamma, TGF beta 1, TNF alpha, OPG, IL-17A, MIP-1 alpha (CCL3), MMP-13, MMP-9, Osteopontin, RANK (TNFRSF11A), CRP, Osteoactivin.
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Tested applicationsSuitable for: Multiplex Protein Detectionmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 2 Membrane 4 Membrane 8 Membrane 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Antibody Arrays 2 units 4 units 8 units Biotinylated Antibody Cocktail 2 vials 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Plastic sheets 1 unit 1 unit 1 unit
Our Abpromise guarantee covers the use of ab211064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
ab211064 has not yet been referenced specifically in any publications.