Overview

  • Product name

    Human Persephin ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 6.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 8.6%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.8 pg/ml
  • Range

    23 pg/ml - 1500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 103 95% - 110%
    Cell culture media 110 103% - 115%
    Heparin Plasma 108 102% - 111%
    EDTA Plasma 78 69% - 84%
    Citrate Plasma 102 97% - 106%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Persephin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Persephin protein in human serum, plasma and cell culture supernatants samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Persephin (PSP) is a neurotrophic factor secreted by glial cell lines. PSP is implicated in cellular death, nervous system development and function; and cellular development and up-regulated in oral squamous cell carcinoma (OSCC)-derived cells. PSP is a glial cell line derived neurotrophic factor (GDNF) which is a member of the transforming growth factor-β superfamily and preferentially binds to the GDNF Receptor alpha 4. The binding of PSP to its receptor activates the tyrosine kinase “rearranged during transfection” or simply known as RET. RET acts to further transduce the signal through several intracellular pathways such as the mitogen-activated protein kinase (MAPK) pathway and the phosphatidylinositol-3-kinase (PI3K) pathway.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Persephin Capture Antibody 1 x 600µl
    10X Human Persephin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human Persephin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 50BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Function

    Exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons.
  • Sequence similarities

    Belongs to the TGF-beta family. GDNF subfamily.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Persephin
    • PSP
    • PSPN
    • PSPN_HUMAN
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Values were interpolated from standard curve and multiplied by dilution factor.

  • Values (mean +/- SD, n = 3) were interpolated from standard curve and multiplied by dilution factor.

Protocols

References

ab193758 has not yet been referenced specifically in any publications.

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