Key features and details
- Sensitivity: 20 pg/ml
- Range: 20.58 pg/ml - 15000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman PF4 ELISA Kit (CXCL4)
See all PF4 kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 20 pg/ml
Range20.58 pg/ml - 15000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 108.2 101% - 116% Serum 122.4 110% - 130% Plasma 119.6 111% - 127%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s PF4 (CXCL4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PF4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human PF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and PF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human PF4 ELISA Kit (CXCL4) (ab189573) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human PF4 (lyophilized) 2 units PF4 Microplate (12 x 8 wells) 1 unit Recombinant Human PF4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionReleased during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsBinds non-covalently to a proteoglycan molecule.
- Information by UniProt
- C-X-C motif chemokine 4
- Chemokine (C X C motif) ligand 4
- Chemokine (CXC motif) ligand 4
SimpleStep ELISA kits
ab100628 has been referenced in 2 publications.
- Dai B et al. lncRNA AWPPH promotes the migration and invasion of glioma cells by activating the TGF-ß pathway. Oncol Lett 18:5923-5929 (2019). PubMed: 31788066
- Yang J et al. Peptidome Analysis Reveals Novel Serum Biomarkers for Children with Autism Spectrum Disorder in China. Proteomics Clin Appl 12:e1700164 (2018). PubMed: 29754444