Human PHD2 / prolyl hydroxylase peptide (ab94889)



  • Nature
  • Amino Acid Sequence
    • Species

Associated products


Our Abpromise guarantee covers the use of ab94889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-PHD2 / prolyl hydroxylase antibody [mAbcam74796] (ab74796)

  • Purity
    70 - 90% by HPLC.

  • Form
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • C1ORF12
    • Chromosome 1 Open Reading Frame 12
    • DKFZp761F179
    • ECYT 3
    • ECYT3
    • Egl 9 family hypoxia inducible factor 1
    • EGL 9 homolog of C. elegans 1
    • EGL nine (C.elegans) homolog 1
    • Egl nine homolog 1
    • Egl nine homolog 1 (C. elegans)
    • Egl nine like protein 1
    • EGLN 1
    • Egln1
    • HIF PH2
    • HIF Prolyl Hydroxylase 2
    • HIF-PH2
    • HIF-prolyl hydroxylase 2
    • HIFP4H 2
    • HIFPH2
    • HPH 2
    • HPH-2
    • HPH2
    • Hypoxia inducible factor prolyl hydroxylase 2
    • Hypoxia-inducible factor prolyl hydroxylase 2
    • ORF13
    • P4H2
    • PHD 2
    • PhD2
    • PNAS 118
    • PNAS 137
    • Prolyl Hydroxylase Domain Containing Protein 2
    • Prolyl hydroxylase domain-containing protein 2
    • Rat Homolog of SM20
    • SM 20
    • SM-20
    • SM20
    • Zinc finger MYND domain containing protein 6
    • ZMYND6
    see all
  • Function
    Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
  • Tissue specificity
    According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle.
  • Involvement in disease
    Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3) [MIM:609820]. ECYT3 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels.
  • Sequence similarities
    Contains 1 Fe2OG dioxygenase domain.
    Contains 1 MYND-type zinc finger.
  • Information by UniProt


ab94889 has not yet been referenced specifically in any publications.

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Ab91612 Mwt is 2049.4 Da
Ab94885 Mwt. 2490 Da

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Please note that these products are not full length proteins; they are peptides that are used to produce ab82500 and ab82560 hence these are called as blocking peptides.

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