Human PI 3 Kinase catalytic subunit gamma / PI3K-gamma peptide (ab22948)

Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      C-VLGIKQGEKHSA
    • Amino acids
      1091 to 1102

Specifications

Our Abpromise guarantee covers the use of ab22948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • Form
    Liquid
  • Additional notes

    Previously labelled as PI 3 Kinase catalytic subunit gamma. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

General Info

  • Alternative names
    • 1 phosphatidylinositol 3 kinase
    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma
    • 5-bisphosphate 3-kinase catalytic subunit gamma isoform
    • p110 gamma
    • p110gamma
    • p120 PI3K
    • p120-PI3K
    • Phosphatidylinositol 3 kinase catalytic 110 kD gamma
    • Phosphatidylinositol 3 kinase gamma, p110 gamma
    • Phosphatidylinositol 3 kinase, catalytic, gamma polypeptide
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit gamma
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma isoform
    • Phosphatidylinositol-4
    • Phosphoinositide 3 kinase catalytic gamma polypeptide
    • Phosphoinositide 3 kinase gamma catalytic subunit
    • PI 3 Kinase catalytic subunit gamma
    • PI3 kinase p110 subunit gamma
    • PI3-kinase subunit gamma
    • PI3CG
    • PI3K
    • PI3K-gamma
    • PI3Kgamma
    • PIK3
    • Pik3cg
    • PK3CG_HUMAN
    • PtdIns-3-kinase subunit gamma
    • PtdIns-3-kinase subunit p110-gamma
    • Serine/threonine protein kinase PIK3CG
    see all
  • Function
    3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
  • Tissue specificity
    Pancreas, skeletal muscle, liver and heart.
  • Pathway
    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
  • Sequence similarities
    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • Information by UniProt

References

ab22948 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question

Hello

Thank you for your reply. Indeed the reference number is ab22048 - my mistake.
Please see below the requested details

Order Details
Antibody code: Ab 22048

Problem
Choose: No signal and Non-specific band Multiple bands

Lot number GR-77209-3

General Information
Antibody storage conditions (temperature/reconstitution etc) Alicuoted upon delivery and stored at -20

Description of the problem (high background, wrong band size, more bands, no band etc.) No bands at the correct molecular weight. (some non specific bands at +-50 and 15kDa). We blotted exactly the same samples with a TLR4 antibody from Santa Cruz and got a band in the human cortex and rat intestine samples.

Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)
Human intervertebral disc
Human cortex
Human mononuclear cells
Human subdermal fat
Rat intestine

Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)
IV disc > 4 different samples lysed with DTT, RIPA buffer or NP-40+EDTA lysis buffer (all with prot. inhibitors)
cortex > RIPA buffer + inh
intestine > RIPA buffer + inh
fat > SDS lysis buffer + inh
all samples heated at 70ºC for 10 min instead of boiling except for intestine -5 minutes at 95ºc

Amount of protein loaded
+-25ug loaded / sample

Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)
Reducing, 7%


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)
1.5h transfer at 50V, blocking 2.5% BSA

Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Ms anti TLR4 antibody 1:200 in TTBS+BSA 1%. 1 h RTP, was with TTBS

Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Anti mouse HRP 1:5000 in TTBS 1h RTP

Detection method (ECL, ECLPlus etc.)
Biorad immunstar western C kit

Positive and negative controls used (please specify)
positive control was human cortex, rat intestine and human mononuclear cells which should definately express the receptor. we have confirmed with a different TLR4 antibody (from santa cruz) that in cortex and intestine there is a band.

Optimization attempts (problem solving)
How many times have you tried the Western?
2

Have you run a "No Primary" control?
No

Do you obtain the same results every time?
Yes
e.g. are the background bands always in the same place?


What steps have you altered?


Additional Notes:


Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

Read More
Answer

Thank you for your message and for providing this further information.

I understand your concerns and reviewing the details it is regrettable the results have not been successful. I appreciate the time you have spent on these experiments and would be pleased to arrange afree of charge replacementor credit note in compensation.

I look forward to hearing from you with details of how you would like to proceed.

Read More

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

Could you confirm that for the product code,this isab22048 TLR4 antibody (ab22948 is not a TLR4 antibody)?

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I can confirm that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. So this antibody shoudl be working in Western blot on human samples. However, we do have manyother TLR4 antibodies available that have also been tested in WB and human. They are on the following search results link:

https://www.abcam.com/index.html?pageconfig=searchresults&search=TLR4&pt=1&sk=react&sv=21&sn=Human&l=3&fViewMore=1

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



Order Details
Antibody code:


Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number

Purchase order number
or preferably Abcam order number:



General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, wrong band size, more bands, no band etc.)


Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


Amount of protein loaded


Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method (ECL, ECLPlus etc.)


Positive and negative controls used (please specify)



Optimization attempts (problem solving)
How many times have you tried the Western?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?


What steps have you altered?


Additional Notes:


Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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