Overview

  • Product name

    Human PIP3E Matched Antibody Pair Kit
    See all IPCEF1 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    125 pg/ml - 8000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human PIP3E.


    Both capture and detector antibodies are rabbit monoclonal antibodies.


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab214573) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human PIP3E Capture Antibody 1 x 100µg 1 x 20µg
    Human PIP3E Detector Antibody 1 x 25µg 1 x 5µg
    Human PIP3E Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Enhances the promotion of guanine-nucleotide exchange by PSCD2 on ARF6 in a concentration-dependent manner.
  • Sequence similarities

    Contains 1 PH domain.
  • Cellular localization

    Cytoplasm. Cell membrane. Translocated with PSCD2 to the plasma membrane upon epidermal growth factor (EGF) stimulation.
  • Information by UniProt
  • Alternative names

    • ICEF1_HUMAN
    • Interaction protein for cytohesin exchange factors 1
    • Interactor protein for cytohesin exchange factors 1
    • Ipcef1
    • Phosphoinositide binding protein PIP3 E
    • Phosphoinositide binding protein PIP3E
    • Phosphoinositide-binding protein PIP3-E
    • RP3402L9
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab218799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab218799 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab218799.
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