Overview

  • Product name

    Human Plasminogen ELISA Kit (PLG)
    See all Plasminogen kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.5%
  • Sample type

    Cell culture supernatant, Serum, Plasma, Tissue
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.3 ng/ml
  • Range

    1.25 ng/ml - 80 ng/ml
  • Recovery

    = 101 %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Plasminogen ELISA kit (PLG) is designed for the quantitative measurement of Plasminogen (PLG) concentrations in Human plasma, serum, urine, saliva, milk, tissue samples, cerebrospinal fluid and cell culture supernatants.


    A Plasminogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Plasminogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Plasminogen captured in plate.


    Get higher sensitivity in only 90 minutes with Human Plasminogen ELISA Kit (ab196262) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Plasminogen Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Plasminogen Microplate (12 x 8 well strips) 1 unit
    Plasminogen Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
    Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
  • Tissue specificity

    Present in plasma and many other extracellular fluids. It is synthesized in the liver.
  • Involvement in disease

    Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa.
  • Sequence similarities

    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 5 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Domain

    Kringle domains mediate interaction with CSPG4.
  • Post-translational
    modifications

    N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
    In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.
  • Cellular localization

    Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
  • Information by UniProt
  • Alternative names

    • Plasmin
    • Plasmin light chain B
    • PLG
    • PLMN_HUMAN
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Plasminogen measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human serum and plasma samples diluted 20000-160000 fold. Other samples diluted 1-100 fold.

Protocols

References

This product has been referenced in:

  • Banville N  et al. A quantitative proteomic approach to identify significantly altered protein networks in the serum of patients with lymphangioleiomyomatosis (LAM). PLoS One 9:e105365 (2014). Sandwich ELISA ; Human . Read more (PubMed: 25133674) »
See 1 Publication for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Abreviews
Review of Abcam Plasminogen Human ELISA Kit:

The Plasminogen ELISA, using the Abcam human ELISA kit Ab108893, Lot GR270028-6, was performed by an experienced laboratory technician at an accredited university. The goal of the experiment was to determine the cross-reactivity for rhesus macaque in both EDTA prepared plasma and in serum samples.

Overall, the kit, like other Abcam ELISA kits, was easy to follow and simple to use.

Our experiment consisted of using EDTA-prepared plasma samples and serum samples from N=3 M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius. Samples were diluted serially in a range from 1:5000 to 1:80000 in the given diluent in duplicate. The rest of the assay was performed per protocol’s instructions.

At 1:80000, all samples were below the range of detection. At 1:40000, most samples were below the range of detection. At 1:20000 a few samples were below the range of detection, but most were in range. 1:5000 and 1:10000 were both in the range of detection for all sample types *subtracted ODs in the 0.6 and 0.5 range respectively). The data presented are the extrapolated values.

Overall, the kit is cross-reactive with both Rhesus plasma and Serum, however we were unable to confirm linearity of detection and value ranges are less than expected.

Abcam user community

Verified customer

Submitted Jun 18 2016

Abreviews
Used the kit for the first time and diluted my plasma samples (citrate plasma) 1:20000 as suggested in the manual.
This dilution was right for almost all of my samples.
Followed the kit's instructions and had my results in about 4h.

Will use it again and can highly recommend it.

Abcam user community

Verified customer

Submitted Aug 21 2014

Answer

The kit will recognize the plasmin A chain. This information is according to the uniprot information given below:

Plasmin heavy chain A: aa 20-580
Plasmin heavy chain A, short form: aa 98-580
Plasmin light chain B: 581-810

http://www.uniprot.org/uniprot/P00747

Read More

Answer

Thank you for contacting us and your interest in our products.

Please let me reassure you that the Plasminogen Human ELISA Kit (ab108893) is suitable for use with serum samples. I am sorry for any confusion caused but this discrepancy, we will be updating the datasheet accordingly. Thank you for bringing this to our attention.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to contact us again.

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Answer

Thank you for contacting us.

We do have a Plasminogen Human ELISA kit as ab108893, but this has not been tested for cross-reactivity with angiostatin.

https://www.abcam.com/Plasminogen-Human-ELISA-Kit-ab108893.html

We do have quite a few anti-plasminogen antibodies, but unfortunately I couldn't find any that have been specifically tested for cross-reactivity with angiostatin. The closest one I could find was ab38157 which states, "No cross-reactivity with other human serum proteins." I will confirm with the lab if this antibody has actually been tested with angiostatin.

https://www.abcam.com/Plasminogen-antibody-8E7-ab38157.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thanks for your enquiry!

Please contact our bulk order specialist, Andrea Gray at mailto:Andrea.Gray@abcam.com, regarding discounts on bulk order purchases.

I hope this is helpful. Please contact me again if you have any further questions.

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Answer

Thank you for your reply.

I am happy to confirm that we offer an ELISA kit for human plasminogen:

ab108893 (Plasminogen Human ELISA Kit)

https://www.abcam.com/index.html?datasheet=108893 (or use the following: https://www.abcam.com/index.html?datasheet=108893).

Please follow the above link to review the datasheet for more information.

I will send a quotation in a separate email.

I wish you a good weekend and good luck for your research.

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