Overview

  • Product name

    Human Platelet Glycoprotein IIb + IIIa ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.2%
  • Sample type

    Plasma, Platelets, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.5 ng/ml
  • Range

    1.25 ng/ml - 40 ng/ml
  • Recovery

    101 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Platelet Glycoprotein IIb + IIIa Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Platelet Glycoprotein IIb+IIIa concentrations in Human platelets, platelet-rich plasma and cell culture lysate.


    A Platelet Glycoprotein llb + llla specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Platelet Glycoprotein llb+llla specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Platelet Glycoprotein llb + llla captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab108851.

Protocols

References

This product has been referenced in:

  • Pletsch-Borba L  et al. Biomarkers of vascular injury in relation to myocardial infarction risk: A population-based study. Sci Rep 9:3004 (2019). Read more (PubMed: 30816120) »
  • Graf ME  et al. Biological reproducibility of circulating P-Selectin, Thrombopoietin, GPIIb/IIIa and Thrombomodulin over one year. Clin Biochem N/A:N/A (2017). ELISA ; Human . Read more (PubMed: 28571971) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry and interest in our products. I am very sorry for my delayed response.

I can confirm that the proper value for GPIIbIIIA in human plasma is about 600 ng/ml.

This kit will allow 1 x 96 tests and the price is € 490.

If you need any further assistance in the future, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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