Product nameHuman PLEKHA4 knockout A549 cell lysate
See all PLEKHA4 kits
Cell line information
Parental cell line: A549
Organism: Homo sapiens
Gene editing information
Editing tool: CRISPR/Cas9
Mutation: 1 bp insertion in exon6 and 2 bp deletion in exon6.
Knockout validation: Confirmed by Sanger sequencing.
Reconstitution instructions: To use as a WB control, resuspend in 45 µL of Sample buffer (40% (w/v) Glycerol, 4% (w/v) Lithium Dodecyl Sulfate, 4% Ficoll 400, 0.025% Phenol Red, 0.025% Brilliant Blue G250, 2 mM EDTA) and 5 µL of DTT to resuspend @ 2mg/ml. Mix well, then boil the sample for 10 minutes before loading it onto the gel.
User storage instructions: Upon receiving, lysate can be diluted with 1 x SDS sample buffer & will be stable at -20°C for 12 months. Long term storage at -80°C.
Knockout validationSanger Sequencing
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Storage instructionsStore at -80°C. Please refer to protocols.
Components 1 kit Human PLEKHA4 knockout A549 cell lysate (Lyophilized) 1 x 100µg Human Wild Type A549 cell lysate (Lyophilized) 1 x 100µg
FunctionBinds specifically to phosphatidylinositol-3-phosphate (PtdIns3P), but not to other phosphoinositides.
Tissue specificityHighly expressed in melanoma. Detected at low levels in heart, skeletal muscle, kidney, liver and small intestine.
Sequence similaritiesContains 1 PH domain.
Cellular localizationCytoplasm. Membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab263300 has not yet been referenced specifically in any publications.