Overview

  • Product name
    Human PLGF ELISA Kit
    See all PLGF kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    1.372 pg/ml - 1000 pg/ml
  • Recovery

    89 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 103.4 75% - 115%
    Serum 88.09 79% - 96%
    Plasma 75.69 68% - 93%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PLGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PLGF in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human PLGF coated on a 96-well plate. Standards and samples are pipetted into the wells and PLGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PLGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Human PLGF (lyophilized) 2 vials
    PLGF Microplate (12 x 8 wells) 1 unit
    Recombinant Human PLGF Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
  • Tissue specificity
    While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Domain
    Isoform PlGF-2 contains a basic insert which acts as a cell retention signal.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.
  • Information by UniProt
  • Alternative names
    • AI854365
    • D12S1900
    • MGC93298
    • Pgf
    • PGFL
    • PIGF
    • Placenta growth factor
    • Placental growth factor
    • Placental growth factor vascular endothelial growth factor related protein
    • PlGF
    • PlGF 2
    • PLGF_HUMAN
    • PlGF2
    • SHGC 10760
    • Vascular endothelial growth factor related protein
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab100629 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100629.

    Protocols

    References

    ab100629 has not yet been referenced specifically in any publications.

    Publishing research using ab100629? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

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    Votes: Highest First

    Inquiry: The protocol doesn't give any indications about sample diluition. I tried the 1:2 diluition, but it's gone on saturation. Same thing for ab119613 and ab100629. Please tell me how to go on. Thank you very much.

    Thank you for your message and I'm sorry to hear you have been having some difficulties.

    Optimal dilution of the sample will usually need to be optimized by the end user. In this case, I would appreciate if you could let me know what type of...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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