Human PLGF ELISA Kit (ab100629)

Submit a review Q&A (1)References (4)
Typical Standard Curve

    Key features and details

    • Sensitivity: 2 pg/ml
    • Range: 1.372 pg/ml - 1000 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human PLGF ELISA Kit
      See all PLGF kits

    • Detection method

      Colorimetric

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 2 pg/ml

    • Range

      1.372 pg/ml - 1000 pg/ml

    • Recovery

      89 %

      Sample specific recovery
      Sample type Average % Range
      Cell culture supernatant 103.4 75% - 115%
      Serum 88.09 79% - 96%
      Plasma 75.69 68% - 93%

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam’s PLGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PLGF in serum, plasma, and cell culture supernatants.

      This assay employs an antibody specific for Human PLGF coated on a 96-well plate. Standards and samples are pipetted into the wells and PLGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PLGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    • Notes

      Optimization may be required with urine samples.

    • Platform

      Microplate

    Properties

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100629.

    References (4)

    Publishing research using ab100629? Please let us know so that we can cite the reference in this datasheet.

    ab100629 has been referenced in 4 publications.

    • Rao SL  et al. Altered level of salivary placental growth factor is associated with preeclampsia. Placenta 90:118-120 (2020). PubMed: 32056542
    • Tan L  et al. Sublethal heat treatment of hepatocellular carcinoma promotes intrahepatic metastasis and stemness in a VEGFR1-dependent manner. Cancer Lett 460:29-40 (2019). PubMed: 31173855
    • Cheung WY  et al. Efficient Nonviral Transfection of Human Bone Marrow Mesenchymal Stromal Cells Shown Using Placental Growth Factor Overexpression. Stem Cells Int 2018:1310904 (2018). PubMed: 30675166
    • Garcés MF  et al. Maternal Serum Meteorin Levels and the Risk of Preeclampsia. PLoS One 10:e0131013 (2015). PubMed: 26121675

    Customer reviews and Q&As

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    Question

    Inquiry: The protocol doesn't give any indications about sample diluition. I tried the 1:2 diluition, but it's gone on saturation. Same thing for ab119613 and ab100629. Please tell me how to go on. Thank you very much.

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    Answer

    Thank you for your message and I'm sorry to hear you have been having some difficulties.

    Optimal dilution of the sample will usually need to be optimized by the end user. In this case, I would appreciate if you could let me know what type of samples you are using, and I can find out some recommended dilutions for you for these kits.

    Thank you for your time. i look forward to hearing from you.

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