Key features and details
- Sensitivity: 1.98 pg/ml
- Range: 0.16 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman PMN Elastase ELISA Kit
See all Neutrophil Elastase kits
Intra-assay Sample n Mean SD CV% Overall 4.8% Inter-assay Sample n Mean SD CV% Overall 5.6%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.16 ng/ml - 10 ng/ml
Sample specific recovery Sample type Average % Range Plasma 104 96% - 110%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s PMN Elastase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human PMN Elastase concentrations in Cell culture supernatants, plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma.
PMN Elastase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated PMN Elastase detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the PMN Elastase amount of sample captured in plate.
The capture antibody in the Human PMN-Elastase ELISA kit is specific to PMN elastase, while the detection antibody is specific to alpha1-PI, hence the kit is specific to the PMN Elastase/alpha1-PI complex.
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests Adhesive Films 2 units Control high 1 vial Control low 1 vial HRP-Conjugate anti-alpha1-PI polyclonal antibody 1 x 16ml Human PMN Elastase - alpha1-PI complex Standard, lyophilized, (10 ng/mL upon reconstitution) 1 vial Microplate coated with polyclonal antibody to Human PMN Elastase (12 x 8 wells) 1 unit Sample Diluent 1 x 50ml Stop Solution 1 x 7ml TMB Substrate Solution 1 x 22ml Wash Buffer Concentrate (10x) 1 x 50ml
FunctionModifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
Tissue specificityBone marrow cells.
Involvement in diseaseDefects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain.
- Information by UniProt
- Bone marrow serine protease
ab119553 has been referenced in 15 publications.
- Miralda I et al. Human Neutrophil Granule Exocytosis in Response to Mycobacterium smegmatis. Pathogens 9:N/A (2020). PubMed: 32075233
- Zaramella P et al. Innate immunity ascertained from blood and tracheal aspirates of preterm newborn provides new clues for assessing bronchopulmonary dysplasia. PLoS One 14:e0221206 (2019). PubMed: 31483807
- Koupenova M et al. The role of platelets in mediating a response to human influenza infection. Nat Commun 10:1780 (2019). PubMed: 30992428
- Thålin C et al. Citrullinated histone H3 as a novel prognostic blood marker in patients with advanced cancer. PLoS One 13:e0191231 (2018). ELISA ; Human . PubMed: 29324871
- Schechter MC et al. Neutrophil extracellular trap (NET) levels in human plasma are associated with active TB. PLoS One 12:e0182587 (2017). ELISA ; Human . PubMed: 28777804