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RecombinantSimpleStep

Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (ab260069)

  • Datasheet
  • SDS
  • Protocol Booklet
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SimpleStep ELISA Protocol Diagram
  • Example of human PNP standard curve in Sample Diluent NS.
  • Interpolated concentrations of native PNP in human serum and plasma samples.
  • Sandwich ELISA - Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (ab260069)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 40 pg/ml
  • Range: 78.13 pg/ml - 5000 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human PNP ELISA Kit (Purine Nucleoside Phosphorylase)
    See all Nucleoside phosphorylase kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 8.3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4.1%
  • Sample type

    Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    40 pg/ml
  • Range

    78.13 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96 85% - 108%
    Hep Plasma 90 82% - 104%
    EDTA Plasma 96 85% - 110%
    Cit plasma 103 90% - 114%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (ab260069) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PNP (Purine Nucleoside Phosphorylase) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PNP (Purine Nucleoside Phosphorylase) with 40 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Purine Nucleoside Phosphorylase (PNP) is a catabolic enzyme which breaks down purine residues by releasing the nucleobase and phosphorylating the ribose residue. It is also used to produce nucleotide monobases as a salvage pathway. PNP plays an important role in the maintenance of T-cells. This kit targets the full-length protein.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human PNP (Purine Nucleoside Phosphorylase) Capture Antibody 1 x 600µl
    10X Human PNP (Purine Nucleoside Phosphorylase) Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BR 1 x 6ml
    Human PNP (Purine Nucleoside Phosphorylase) Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
  • Involvement in disease

    Defects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNP deficiency) [MIM:613179]. It leads to a severe T-cell immunodeficiency with neurologic disorder in children.
  • Sequence similarities

    Belongs to the PNP/MTAP phosphorylase family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P00491 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • FLJ94043
    • FLJ97288
    • FLJ97312
    • Inosine phosphorylase
    • Inosine-guanosine phosphorylase
    • MGC117396
    • MGC125915
    • MGC125916
    • NP
    • Np1
    • Nucleoside phosphorylase
    • PNP
    • Pnp1
    • PNPH_HUMAN
    • PRO1837
    • PUNP
    • Purine nucleoside orthophosphate ribosyltransferase
    • Purine nucleoside phosphorylase
    • Purine nucleoside phosphorylase 5a
    see all
  • Database links

    • Entrez Gene: 4860 Human
    • Omim: 164050 Human
    • SwissProt: P00491 Human
    • Unigene: 75514 Human

    Associated products

    • Alternative Versions

      • Human PNP (Purine Nucleoside Phosphorylase) Antibody Pair - BSA and Azide free (ab267692)

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Example of human PNP standard curve in Sample Diluent NS.
      Example of human PNP standard curve in Sample Diluent NS.

      The PNP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native PNP in human serum and plasma samples.
      Interpolated concentrations of native PNP in human serum and plasma samples.

      The concentrations of PNP were measured in duplicate, interpolated from the PNP standard curves, and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (edta) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PNP concentration was determined to be 1435.64 pg/mL in serum, 4419.64 pg/mL in plasma (citrate), 2483.57 pg/mL in plasma (heparin), and 1750.71 pg/mL in plasma (edta).

    • Sandwich ELISA - Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (ab260069)
      Sandwich ELISA - Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (ab260069)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab260069? Please let us know so that we can cite the reference in this datasheet.

    ab260069 has not yet been referenced specifically in any publications.

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