For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    human-polyoma-virus-jcv-capsid-protein-vp1-antibody-8e8-ab34756.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Microbiology Organism Virus DNA Virus double stranded DNA Virus Other dsDNA viruses
Share by email

Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (ab34756)

  • Datasheet
  • SDS
Reviews (2)Q&A (8)References (12)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (ab34756)

    Key features and details

    • Mouse monoclonal [8E8] to Human Polyoma virus JCV capsid protein VP1
    • Suitable for: Indirect ELISA, WB
    • Isotype: IgG2a

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Primary
    Product image
    Alexa Fluor® 488 Anti-CRISPR-Cas9 antibody [EPR18991] (ab215239)
    Primary
    Product image
    Anti-Clathrin heavy chain antibody (ab21679)

    View more associated products

    Overview

    • Product name

      Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8]
    • Description

      Mouse monoclonal [8E8] to Human Polyoma virus JCV capsid protein VP1
    • Host species

      Mouse
    • Tested applications

      Suitable for: Indirect ELISA, WBmore details
    • Species reactivity

      Reacts with: Other species
    • Immunogen

      Recombinant full length protein corresponding to Human Polyoma virus JCV capsid protein VP1. Recombinant full length purified major capsid protein VP1 of human polyomavirus JCV expressed in yeast S.cerevisiae.

    • General notes

      This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR48370-3, GR185137-5, GR185137-7 and GR185137-8 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.2
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      8E8
    • Myeloma

      Sp2/0
    • Isotype

      IgG2a
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • DNA Virus
      • double stranded DNA Virus
      • Other dsDNA viruses

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab34756 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA
    1/1000 - 1/10000.
    WB
    1/1000 - 1/5000.
    Notes
    Indirect ELISA
    1/1000 - 1/10000.
    WB
    1/1000 - 1/5000.

    Target

    • Relevance

      The human polyomavirus JC virus (JCV) infects greater than 80% of the human population. The JC virus is a small (38-40 nm in diameter) double stranded, circular DNA virus covered by an icosahedral capsid. Infection with JCV is asymptomatic and it occurs in early childhood. After the primary infection, the virus remains in latent state in the kidney, until it's reactivation under immunosuppressive conditions to result in Progressive Multifocal Leukoencephalopathy (PML), a fatal demyelinating disease. 70% of all HIV-1- infected patients will exhibit neurological disorders and between 5 and 8% of all HIV-1-infected patients will develop PML. Similar to other polyomaviruses, JCV can cause tumors when intracerebrally inoculated at high titers into developing rodent. Several reports suggest the association of viruses, especially of the polyomavirus family with different types of human brain tumors. Tumorigenecity of JCV is most likely induced by the viral early gene product T-antigen. T-antigen has the capacity to interact with several tumor suppressor proteins, most notably p53, and functionally inactivate these proteins.
    • Database links

      • Entrez Gene: 2828425 Other species

      Images

      • Western blot - Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (ab34756)
        Western blot - Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (ab34756)

        Immunoblot of recombinant major capsid protein VP1 (500 ng per lane) of human polyomavirus JCV using monoclonal antibody ab34756 at a concentration of 1 µg/mL.

      Protocols

      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (12)

      Publishing research using ab34756? Please let us know so that we can cite the reference in this datasheet.

      ab34756 has been referenced in 12 publications.

      • Scribano S  et al. Archetype JC polyomavirus DNA associated with extracellular vesicles circulates in human plasma samples. J Clin Virol 128:104435 (2020). PubMed: 32442760
      • Wilczek MP  et al. JC Polyomavirus Infection Reveals Delayed Progression of the Infectious Cycle in Normal Human Astrocytes. J Virol 94:N/A (2020). PubMed: 31826993
      • Querido S  et al. High-grade urothelial carcinoma in a kidney transplant recipient after JC virus nephropathy: The first evidence of JC virus as a potential oncovirus in bladder cancer. Am J Transplant 20:1188-1191 (2020). PubMed: 31654479
      • Reoma LB  et al. Fatal encephalopathy with wild-type JC virus and ruxolitinib therapy. Ann Neurol 86:878-884 (2019). PubMed: 31600832
      • DuShane JK  et al. High-Throughput Characterization of Viral and Cellular Protein Expression Patterns During JC Polyomavirus Infection. Front Microbiol 10:783 (2019). PubMed: 31065251
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 10 Abreviews or Q&A

      Immunohistochemistry free floating abreview for Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry free floating
      Sample
      Human Tissue sections (brain)
      Specification
      brain
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 29 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Human Polyoma virus JCV capsid protein VP1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Fetal glial cell (SVG-A))
      Permeabilization
      No
      Specification
      Fetal glial cell (SVG-A)
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
      Fixative
      Methanol
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 24 2015

      Question

      Inquiry: I would like to use this antibody (ab34756) for ELISA. Could you please suggest a product to use as a positive control and construct standard curves?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 16 2013

      Answer

      Thank you for your interest in Abcam.

      We unfortunately do not sell this product, Human Polyoma virus JCV. This product however might be available from different companies. Please check it online. You can also try searching in bio compare website at http://www.biocompare.com/, this webiste has good search facility for different product types.


      I hope this information will be helpful. Please do not hesitate to contact me if you have any questions.

      Read More

      Abcam Scientific Support

      Answered on Apr 16 2013

      Question


      Wir wollen eine ELISA für humane Polyoma IgG's und IgM's aufbauen. Da ich kein Kit finde bin ich auf Ihre Polyoma Proteine und Antikörper aufmerksam geworden.

      Habe ich es richtig verstanden, dass das folgende Protein
      JCV Polyomavirus Major Capsid VP1 protein (ab 74569)
      einem humanem Polyomavirus entspricht? In den References wird zumindestens auf human eingegangen?
      Was ist der unterschied zum
      BK polyomavirus, strain AS, Major Capsid VP1 protein (ab74567)?
      Wird dieses Protein auch von humanen IgG's und IgM's detektiert?

      Da wir auch eine Positivkontrolle brauchen habe ich den Antikörper
      Anti-Human Polyoma virus JCV capsid protein VP1 antibody (ab34756) rausgesucht.
      Doch für die cross reactivity ist angegeben, das der Antikörper auf Hamster und nicht auf humane Polyomaviren reagiert. Das verwirrt mich etwas.
      Beim Anti-Hamster Polyoma virus major capsid protein VP1 antibody (ab 34755) wird als alternativer Name "Coat protein VP1 antoibody" angegeben. Ist dieser als Positivkontrolle besser geeignet?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 22 2013

      Answer



      Die Spezifitätsnotiz beschreibt folgendes:

      ab34756 bindet das Major Capsidprotein VP1 von humanem Polyoma Virus JCV und das Capsidprotein VP1 von hamster polyomavirus (HaPyV) and Simian Virus 40 (SV40).

      ab34756 kreuz-reagiert nicht mit dem Major Capsidprotein VP1 von humanem polyomavirus BKV (anderer Stamm) und murinem polyomavirus (MuPyV).

      Ich musste es auch dreimal lesen :-)

      D.H., dass ab34756 mit ab74569 (JCV Polyomavirus Major Capsid VP1 protein) als Positivkontrolle verwendet werden kann.

      Bitte beachten Sie, dass ab34756 ein Mausantikörper ist und keine direkten Vergleiche mit humanem IgG oder IgM gezogen werden können. Als Test ob der ELISA grundsätzlich funktioniert ist er aber geeignet.

      Read More

      Abcam Scientific Support

      Answered on Mar 22 2013

      Question

      Can I have more informations about the epitope recognized by this monoclonal antibody?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 09 2013

      Answer

      ab34756 recognizes the C-terminal part of human polyomavirus JCV capsid protein VP1. The epitope is localized between 290 and 384 aa residues of JCV capsid protein VP1.

      Read More

      Abcam Scientific Support

      Answered on Jan 09 2013

      Question

      I'm happy to test the antibody and send you a review. So, please give me a
      discount code. There is no rush.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 01 2012

      Answer

      DISCOUNT CODE: ***********
      Expiration date: **************
      I am very pleased to hear you would like to accept our offer and test ab34756 in IHC. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for IHC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Mar 01 2012

      Question

      I am looking for an anti-JC virus VP1 antibody that works in immunocytochemistry/histochemistry; and the host should be other than rabbit.
      I was wondering if your product, ab34756 has been tested for staining by now. I would appreciate any information.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 01 2012

      Answer

      Thank you very much for your interest in ab34756.
      To our knowledge, ab34756 has not been tested in IHC. Therefore, I can offer a discount off a future purchase if you buy ab34756 now, test it in IHC and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.
      If you are interested in this offer, please follow these steps:
      1. Reply to this e-mail to let me know that you would like to proceed and test ab34756 in IHC, I will then send a discount code. This code must be issued before purchasing ab34756 so please wait for my reply before ordering.
      2. Purchase ab34756 either by phone, fax, or online (www.abcam.com).
      3. Test it in IHC.
      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab34756 turns out to be unsuitable for IHC, you will still receive the discount on your next purchase after your Abreview has been submitted.
      Please let me know if you have any questions about this offer and I would be happy to help you further.
      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Mar 01 2012

      Question

      Can I also detect ab74565 (SV40 VP1) with this mAb ab34756 (anti-Hu JCV)? Please confirm it.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 13 2012

      Answer

      Thank you for your reply. I can confirm that the antibody ab34756 will react with the SV40 VP1 protein ab74565. On the antibody datasheet in the section labelled "cross-reactivity", it notes that it does react with the VP1 protein of SV40. I hope this will give you some reassurance. Please let me know if you have any additional questions and I'll be happy to help.

      Read More

      Abcam Scientific Support

      Answered on Jan 13 2012

      Question

      Are these proteins tagged?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 12 2012

      Answer

      Thank you for your call this week and for your patience while I have been in touch with the production lab about these proteins. I have confirmed with the lab that these proteins do not contain any tag. To detect them, you could use specific monoclonal antibody ab34756, which reacts with both proteins- https://www.abcam.com/Human-Polyoma-virus-JCV-capsid-protein-VP1-antibody-ab34756.html I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

      Read More

      Abcam Scientific Support

      Answered on Jan 12 2012

      Question

      Request for testing discount: ICC/IF (IF detection on captured virus)

      Read More

      Abcam community

      Verified customer

      Asked on Oct 11 2011

      Answer

      DISCOUNT CODE: ****** Expiration date: February 11, 2012 I am very pleased to hear you would like to accept our offer and test ab34756 in ICC/IF. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Oct 11 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2022 Abcam plc. All rights reserved.