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Recombinant full length protein corresponding to Human Polyoma virus JCV capsid protein VP1. Recombinant full length purified major capsid protein VP1 of human polyomavirus JCV expressed in yeast S.cerevisiae.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR48370-3, GR185137-5, GR185137-7 and GR185137-8 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Our Abpromise guarantee covers the use of ab34756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||1/1000 - 1/10000.|
|WB||1/1000 - 1/5000.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 25155602|
Immunoblot of recombinant major capsid protein VP1 (500 ng per lane) of human polyomavirus JCV using monoclonal antibody ab34756 at a concentration of 1 µg/mL.
ab34756 staining Human Polyoma virus JCV capsid protein VP1 in human fetal glial cell (SVG-A) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 2% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000 in PBS + BSA) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"