Overview

  • Product name

    Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8]
  • Description

    Mouse monoclonal [8E8] to Human Polyoma virus JCV capsid protein VP1
  • Host species

    Mouse
  • Tested applications

    Suitable for: Indirect ELISA, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Recombinant full length protein corresponding to Human Polyoma virus JCV capsid protein VP1. Recombinant full length purified major capsid protein VP1 of human polyomavirus JCV expressed in yeast S.cerevisiae.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR48370-3, GR185137-5, GR185137-7 and GR185137-8 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    8E8
  • Myeloma

    Sp2/0
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/1000 - 1/10000.
WB 1/1000 - 1/5000.
ICC/IF Use at an assay dependent concentration. PubMed: 25155602

Target

  • Relevance

    The human polyomavirus JC virus (JCV) infects greater than 80% of the human population. The JC virus is a small (38-40 nm in diameter) double stranded, circular DNA virus covered by an icosahedral capsid. Infection with JCV is asymptomatic and it occurs in early childhood. After the primary infection, the virus remains in latent state in the kidney, until it's reactivation under immunosuppressive conditions to result in Progressive Multifocal Leukoencephalopathy (PML), a fatal demyelinating disease. 70% of all HIV-1- infected patients will exhibit neurological disorders and between 5 and 8% of all HIV-1-infected patients will develop PML. Similar to other polyomaviruses, JCV can cause tumors when intracerebrally inoculated at high titers into developing rodent. Several reports suggest the association of viruses, especially of the polyomavirus family with different types of human brain tumors. Tumorigenecity of JCV is most likely induced by the viral early gene product T-antigen. T-antigen has the capacity to interact with several tumor suppressor proteins, most notably p53, and functionally inactivate these proteins.
  • Database links

    Images

    • Immunoblot of recombinant major capsid protein VP1 (500 ng per lane) of human polyomavirus JCV using monoclonal antibody ab34756 at a concentration of 1 µg/mL.

    • ab34756 staining Human Polyoma virus JCV capsid protein VP1 in human fetal glial cell (SVG-A) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 2% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000 in PBS + BSA) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:

    • DuShane JK  et al. High-Throughput Characterization of Viral and Cellular Protein Expression Patterns During JC Polyomavirus Infection. Front Microbiol 10:783 (2019). Read more (PubMed: 31065251) »
    • Diotti RA  et al. Divergent Trends of Anti-JCPyV Serum Reactivity and Neutralizing Activity in Multiple Sclerosis (MS) Patients during Treatment with Natalizumab. Viruses 8:N/A (2016). Read more (PubMed: 27164128) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    1-9 of 9 Abreviews or Q&A

    Answer

    Thank you for your interest in Abcam.

    We unfortunately do not sell this product, Human Polyoma virus JCV. This product however might be available from different companies. Please check it online. You can also try searching in bio compare website at http://www.biocompare.com/, this webiste has good search facility for different product types.


    I hope this information will be helpful. Please do not hesitate to contact me if you have any questions.

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    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Fetal glial cell (SVG-A))
    Permeabilization
    No
    Specification
    Fetal glial cell (SVG-A)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Methanol

    Abcam user community

    Verified customer

    Submitted Jul 24 2015

    Answer



    Die Spezifitätsnotiz beschreibt folgendes:

    ab34756 bindet das Major Capsidprotein VP1 von humanem Polyoma Virus JCV und das Capsidprotein VP1 von hamster polyomavirus (HaPyV) and Simian Virus 40 (SV40).

    ab34756 kreuz-reagiert nicht mit dem Major Capsidprotein VP1 von humanem polyomavirus BKV (anderer Stamm) und murinem polyomavirus (MuPyV).

    Ich musste es auch dreimal lesen :-)

    D.H., dass ab34756 mit ab74569 (JCV Polyomavirus Major Capsid VP1 protein) als Positivkontrolle verwendet werden kann.

    Bitte beachten Sie, dass ab34756 ein Mausantikörper ist und keine direkten Vergleiche mit humanem IgG oder IgM gezogen werden können. Als Test ob der ELISA grundsätzlich funktioniert ist er aber geeignet.

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    Question
    Answer

    ab34756 recognizes the C-terminal part of human polyomavirus JCV capsid protein VP1. The epitope is localized between 290 and 384 aa residues of JCV capsid protein VP1.

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    Answer

    DISCOUNT CODE: ***********
    Expiration date: **************
    I am very pleased to hear you would like to accept our offer and test ab34756 in IHC. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for IHC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Answer

    Thank you very much for your interest in ab34756.
    To our knowledge, ab34756 has not been tested in IHC. Therefore, I can offer a discount off a future purchase if you buy ab34756 now, test it in IHC and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.
    If you are interested in this offer, please follow these steps:
    1. Reply to this e-mail to let me know that you would like to proceed and test ab34756 in IHC, I will then send a discount code. This code must be issued before purchasing ab34756 so please wait for my reply before ordering.
    2. Purchase ab34756 either by phone, fax, or online (www.abcam.com).
    3. Test it in IHC.
    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab34756 turns out to be unsuitable for IHC, you will still receive the discount on your next purchase after your Abreview has been submitted.
    Please let me know if you have any questions about this offer and I would be happy to help you further.
    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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    Answer

    Thank you for your reply. I can confirm that the antibody ab34756 will react with the SV40 VP1 protein ab74565. On the antibody datasheet in the section labelled "cross-reactivity", it notes that it does react with the VP1 protein of SV40. I hope this will give you some reassurance. Please let me know if you have any additional questions and I'll be happy to help.

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    Question
    Answer

    Thank you for your call this week and for your patience while I have been in touch with the production lab about these proteins. I have confirmed with the lab that these proteins do not contain any tag. To detect them, you could use specific monoclonal antibody ab34756, which reacts with both proteins- https://www.abcam.com/Human-Polyoma-virus-JCV-capsid-protein-VP1-antibody-ab34756.html I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

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    Question
    Answer

    DISCOUNT CODE: ****** Expiration date: February 11, 2012 I am very pleased to hear you would like to accept our offer and test ab34756 in ICC/IF. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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