Overview

  • Product name

    Human PR3 ELISA Kit
    See all PR3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 8 3.7%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 3 11%
  • Sample type

    Cell culture supernatant, Cell culture extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    150 pg/ml
  • Range

    1.2 ng/ml - 150 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101 90% - 117%
    Cell culture extracts 89 87% - 93%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    PR3 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PR3 protein in Human cell extracts and cell supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Human PR3 is a polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.


    Sensitivity:


    Samples diluted in Sample Diluent NS - 150pg/mL.


    Samples diluted in Cell Extraction Buffer PTR - 641.8 pg/mL.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    10X Human PR3 Capture Antibody 1 x 600µl
    10X Human PR3 Detector Antibody 1 x 600µl
    Human PR3 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
  • Sequence similarities

    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Alternative names

    • ACPA
    • AGP 7
    • AGP7
    • AGP7 serine proteinase
    • Azurophil Granule Protein 7
    • C ANCA
    • C ANCA antigen
    • C-ANCA antigen
    • CANCA
    • EC 3.4.21.76
    • Leukocyte proteinase 3
    • MBN
    • MBT
    • MBT WEGENER AUTOANTIGEN
    • Myeloblastin
    • Neutrophil proteinase 4
    • NP 4
    • NP-4
    • NP4
    • P29
    • PR 3
    • PR-3
    • PR3
    • Proteinase 3
    • Proteinase3
    • PRTN 3
    • Prtn3
    • PRTN3_HUMAN
    • Serine proteinase neutrophil Wegener granulomatosis autoantigen
    • Serine proteinase, neutrophil
    • Wegener autoantigen
    • Wegener granulomatosis autoantigen
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab226902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of PR3 were measured in duplicates, interpolated from the PR3 standard curves and corrected for sample dilution. Undiluted samples are as follows: THP-1 cell extract (100 g/ml); HL-60 (50 g/ml); untreated THP-1 cell supernatant (100 %) and untreated Human PBMC supernatant (50%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PR3 concentration was determined to be 24.62 ng/mL in THP-1 cell extract, 72.65 ng/mL in HL-60 cell extract and 6.16 ng/mL in THP-1 supernatant and 146.21 ng/ml in PBMC supernatant.

Protocols

References

ab226902 has not yet been referenced specifically in any publications.

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