• Product name
    Human PreAlbumin ELISA Kit (Transthyretin)
    See all Prealbumin kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 4.3%
    Sample n Mean SD CV%
    Overall 9.9%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 55 pg/ml
  • Range
    0.122 ng/ml - 31.25 ng/ml
  • Recovery

    = 98 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human PreAlbumin (Transthyretin) ELISA  (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PreAlbumin concentrations in plasma, serum, urine, saliva, milk and cell culture supernatants.

    A PreAlbumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PreAlbumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PreAlbumin captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human PreAlbumin Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    PreAlbumin Microplate (12 x 8 well strips) 1 unit
    PreAlbumin Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • Tissue specificity
    Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  • Involvement in disease
    Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
    Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
    Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
  • Sequence similarities
    Belongs to the transthyretin family.
  • Domain
    Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
  • Cellular localization
    Secreted. Cytoplasm.
  • Information by UniProt
  • Alternative names
    • Amyloid polyneuropathy
    • Amyloidosis I
    • ATTR
    • Carpal tunnel syndrome 1
    • CTS
    • CTS1
    • Dysprealbuminemic euthyroidal hyperthyroxinemia
    • Dystransthyretinemic hyperthyroxinemia
    • Epididymis luminal protein 111
    • HEL111
    • HsT2651
    • PALB
    • Prealbumin
    • Prealbumin amyloidosis type I
    • Prealbumin Thyroxine-binding
    • Senile systemic amyloidosis
    • TBPA
    • Thyroxine binding prealbumin
    • Transthyretin
    • TTR
    • TTR protein
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.


  • Standard curve of Hu PreAlbumin, with background signal subtracted (duplicates; +/- SD).
  • PreAlbumin measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • PreAlbumin detected in cell supernatants, results shown after background signal was subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108895.



This product has been referenced in:
  • Murakami Y  et al. Spontaneous intracranial hypotension is diagnosed by a combination of lipocalin-type prostaglandin D synthase and brain-type transferrin in cerebrospinal fluid. Biochim Biophys Acta 1862:1835-1842 (2018). Read more (PubMed: 29621631) »
  • Ambrosius W  et al. Predictive value of serum transthyretin for outcome in acute ischemic stroke. PLoS One 12:e0179806 (2017). Read more (PubMed: 28636639) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


The 10x diluent can have usual particulate that forms. These are simple salt deposits that the process of diluting breaks up and dissolves and there is no worry that this will affect results from the kit as you have also seen in your experiments.

I would definitely recommend using a fresh standard for each plate. The protein for this kit is highly unstable once it has been reconstituted, and we routinely only use it for 2 – 3 days before we begin to see a drop in its effectiveness. Even storing it over the weekend at -20C tends to result in lower OD’s.

The biotinylated antibody and SP conjugate are fine up to the end of the guarantee period (12 months after purchase) of the kit itself so long as they have been stored at -20C. They are both in a stabilizing solution that shouldn’t permit them to freeze, we have managed to test some of our biotins 4 or 5 years after their production without any appreciable change in performance.

It is only the standard that needs to be treated very carefully in this kit and that is the reason why we include two separate vials of standard.

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You should use the protocol supplied in the box with the kit as the method is optimized for that batch of kit components.
In this case the difference relates mainly to extra information supplied with lot 123855 and minor differences in incubation times between the sets of reagents.
When multiple kits are ordered, we attempt to send all kits from the same lot. In this instance, at the time of your order we did not have enough kits of the same lot to fulfill your order, so 2 different lots were sent, and the specific protocols sent with the respective lot should be followed.

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Our source for this assay claims "the kit will measure total prealbumin level" and that "it can recognize all forms of prealbumin".

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Thank you very much for contacting us with your question, and for your patience while I have been in touch with the lab regarding your enquiry.

This kit has been tested with samples from many different species, including bovine, monkey, mouse, rat, and canine, but no species cross-reactivity has been found. This kit is very specific for the human prealbumin and we don't expect it to cross-react with polar bear samples, unfortunately.

I hope that this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you, and I'll be happy to help.

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