Overview

  • Product name

    Human Prekallikrein 1B ELISA Kit, Fluorescent
    See all Plasma Kallikrein 1B kits
  • Detection method

    Fluorescent
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 6.3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.8%
  • Sample type

    Cell culture supernatant, Milk, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    76 pg/ml
  • Range

    0.12 ng/ml - 60 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94 87% - 101%
    Milk 85 82% - 91%
    Serum 92 84% - 98%
    Hep Plasma 91 87% - 97%
    EDTA Plasma 101 88% - 107%
    Cit plasma 92 87% - 95%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Sheep, Goat, Cow, Pig
  • Product overview

    Prekallikrein 1B in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Prekallikrein and Plasma Kallikrein 1B protein in human serum, plasma, milk, and cell culture supernatant.


    This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
    If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.


    The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

  • Notes

    Prekallikrein 1B (KLKB1) is a 88 kDa serine protease involved in blood coagulation, kinin generation, fibrinolysis and inflammation. Mature KLKB1 primarily circulates as a complex with high-molecular weight kinnogen, where it releases kinins from kininogens during the inflammation and vasodilation response.

    This kit detects both prekallikrein and plasma kallikrein.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Prekallikrein 1B Capture Antibody 1 x 600µl
    10X Human Prekallikrein 1B Detector Antibody 1 x 600µl
    Human Prekallikrein 1B Lyophilized Purified Protein 2 vials
    Antibody Diluent 4BI 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Stoplight Red Substrate Buffer 1 x 12ml
    100X Stoplight Red Substrate 1 x 120µl
    500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated Black 96-Well Microplate 1 unit
    Plate Seals 1 unit
  • Research areas

  • Function

    The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • Involvement in disease

    Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
  • Sequence similarities

    Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Fletcher factor
    • kallikrein B plasma
    • Kallikrein B plasma (Fletcher factor) 1
    • Kallikrein B, plasma 1
    • Kallikrein B1
    • Kininogenin
    • KLK3
    • KLKB1
    • KLKB1_HUMAN
    • PKK
    • PKKD
    • Plasma kallikrein
    • Plasma kallikrein B1
    • Plasma kallikrein light chain
    • Plasma prekallikrein
    • PPK
    • Prekallikrein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab229411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Serum from 10 healthy male donors was measured in duplicate. The mean Plasma Kallikrein 1B concentration was determined to be 112.6 µg/mL with a range of 80.8 – 206.4 µg/mL in male donors.

Protocols

References

ab229411 has not yet been referenced specifically in any publications.

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