Human Prekallikrein and Kallikrein ELISA Kit (ab171015)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Human Prekallikrein and Kallikrein ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall = 4.3% Inter-assay Sample n Mean SD CV% Overall = 8.9% -
Sample type
Cell culture supernatant, Saliva, Milk, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
> 1.2 ng/ml -
Range
1.25 ng/ml - 80 ng/ml -
Recovery
= 98 %
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Assay time
4h 00m -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Prekallikrein and Kallikrein Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Prekallikrein and Kallikrein levels in plasma, serum, saliva, milk, cerebrospinal fluid and cell culture supernatants.
A Prekallikrein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Prekallikrein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Prekallikrein and Kallikrein captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Tested applications
Suitable for: Sandwich ELISAmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Prekallikrein Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Prekallikrein Microplate (12 x 8 well strips) 1 unit Prekallikrein Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. -
Involvement in disease
Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. -
Sequence similarities
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Fletcher factor
- Kininogenin
- KLKB1
see all -
Database links
- Entrez Gene: 3818 Human
- Omim: 229000 Human
- SwissProt: P03952 Human
- Unigene: 237642 Human
Applications
Our Abpromise guarantee covers the use of ab171015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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ab171015 Prekallikrein and Kallikrein Human ELISA KitPrekallikrein measured in various biologicals showing mass (ng) per mL of sample tested.
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Typical Standard CurveRepresentative Standard Curve using ab171015
Protocols
References
This product has been referenced in:
- Harbaum L et al. Reduced plasma levels of small HDL particles transporting fibrinolytic proteins in pulmonary arterial hypertension. Thorax 74:380-389 (2019). Read more (PubMed: 30478197) »
