Overview

  • Product name

    Human Prekallikrein and Kallikrein ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall = 4.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall = 8.9%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    > 1.2 ng/ml
  • Range

    1.25 ng/ml - 80 ng/ml
  • Recovery

    = 98 %

  • Assay time

    4h 00m
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Prekallikrein and Kallikrein Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Prekallikrein and Kallikrein levels in plasma, serum, saliva, milk, cerebrospinal fluid and cell culture supernatants.


    A Prekallikrein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Prekallikrein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Prekallikrein and Kallikrein captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab171015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Prekallikrein measured in various biologicals showing mass (ng) per mL of sample tested.

  • Representative Standard Curve using ab171015

Protocols

References

This product has been referenced in:

  • Harbaum L  et al. Reduced plasma levels of small HDL particles transporting fibrinolytic proteins in pulmonary arterial hypertension. Thorax 74:380-389 (2019). Read more (PubMed: 30478197) »
See 1 Publication for this product

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