Human Prekallikrein ELISA Kit (ab171015)

Precision

Intra-assay
Sample	n	Mean	SD	CV%
Overall				= 4.3%

Inter-assay
Sample	n	Mean	SD	CV%
Overall				= 8.9%

Sample type

Cell culture supernatant, Saliva, Milk, Serum, Plasma
Assay type

Sandwich (quantitative)
Sensitivity

> 1.2 ng/ml
Range

1.25 ng/ml - 80 ng/ml
Recovery

= 98 %
Assay time

4h 00m
Species reactivity

Reacts with: Human
Product overview

Abcam’s Prekallikrein and Kallikrein Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Prekallikrein and Kallikrein levels in plasma, serum, saliva, milk, cerebrospinal fluid and cell culture supernatants.
A Prekallikrein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Prekallikrein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Prekallikrein and Kallikrein captured in plate.
Tested applications

Suitable for: Sandwich ELISAmore details

Components	1 x 96 tests
100X Streptavidin-Peroxidase Conjugate	1 x 80µl
10X Diluent N Concentrate	1 x 30ml
20X Wash Buffer Concentrate	2 x 30ml
50X Biotinylated Human Prekallikrein Antibody	1 x 120µl
Chromogen Substrate	1 x 8ml
Prekallikrein Microplate (12 x 8 well strips)	1 unit
Prekallikrein Standard	1 vial
Sealing Tapes	3 units
Stop Solution	1 x 12ml

Research areas
Function

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Involvement in disease

Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
Sequence similarities

Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain.
Cellular localization

Secreted.
Target information above from: UniProt accession P03952 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Alternative names
- Fletcher factor
- Kininogenin
- KLKB1
- KLKB1_HUMAN
- Plasma kallikrein light chain
- Plasma prekallikrein
see all
Database links
- Entrez Gene: 3818 Human
- Omim: 229000 Human
- SwissProt: P03952 Human
- Unigene: 237642 Human

Our Abpromise guarantee covers the use of ab171015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Sandwich ELISA		Use at an assay dependent concentration.

ab171015 Prekallikrein and Kallikrein Human ELISA Kit

Prekallikrein measured in various biologicals showing mass (ng) per mL of sample tested.
Typical Standard Curve

Representative Standard Curve using ab171015

ab171015 has not yet been referenced specifically in any publications.

Publishing research using ab171015? Please let us know so that we can cite the reference in this datasheet.

Human Prekallikrein and Kallikrein ELISA Kit (ab171015)