Human Prekallikrein and Kallikrein ELISA Kit (ab171015)
- Datasheet
- References
- Protocols
Overview
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Product nameHuman Prekallikrein and Kallikrein ELISA Kit
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Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall = 4.3% Inter-assay Sample n Mean SD CV% Overall = 8.9% -
Sample typeCell culture supernatant, Saliva, Milk, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity> 1.2 ng/ml
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Range1.25 ng/ml - 80 ng/ml
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Recovery
= 98 %
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Assay time4h 00m
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Species reactivityReacts with: Human
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Product overview
Abcam’s Prekallikrein and Kallikrein Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Prekallikrein and Kallikrein levels in plasma, serum, saliva, milk, cerebrospinal fluid and cell culture supernatants.
A Prekallikrein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Prekallikrein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Prekallikrein and Kallikrein captured in plate.
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Tested applicationsSuitable for: Sandwich ELISAmore details
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Prekallikrein Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Prekallikrein Microplate (12 x 8 well strips) 1 unit Prekallikrein Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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FunctionThe enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
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Involvement in diseaseDefects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect.
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Sequence similaritiesBelongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain. -
Cellular localizationSecreted.
- Information by UniProt
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Alternative names
- Fletcher factor
- Kininogenin
- KLKB1
see all -
Database links
- Entrez Gene: 3818 Human
- Omim: 229000 Human
- SwissProt: P03952 Human
- Unigene: 237642 Human
Applications
Our Abpromise guarantee covers the use of ab171015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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ab171015 Prekallikrein and Kallikrein Human ELISA KitPrekallikrein measured in various biologicals showing mass (ng) per mL of sample tested.
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Typical Standard CurveRepresentative Standard Curve using ab171015
Protocols
References
ab171015 has not yet been referenced specifically in any publications.
