Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit (ab216064)

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Overview

  • Product name

    Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit
    See all Procollagen Type 1 kits

  • Detection method

    Colorimetric

  • Assay type

    ELISA set

  • Sensitivity

    8.44 pg/ml

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Pro-Collagen I alpha 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Pro-Collagen I alpha 1.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210966. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.


    We’ve listened to you: due to popular demand, we will now provide our Matched Antibody Pair kits in 5x96 tests and 10x96 tests. The 2x96 tests size will be discontinued on 30th June 2020 unless inventory is depleted beforehand.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests 5 x 96 tests
    Human Pro-Collagen I alpha 1 Capture Antibody 1 x 100µg 1 x 20µg 1 x 50µg
    Human Pro-Collagen I alpha 1 Detector Antibody 1 x 25µg 1 x 5µg 1 x 12.5µg
    Human Pro-Collagen I alpha 1 Lyophilized Protein 1 vial 1 vial 1 vial

  • Research areas

  • Relevance

    Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Procollagen type 1 is mainly synthesized by osteoblasts and, after cleavage of the N- and C-terminal extension peptides, is utilized for collagen fibril deposition in the osteoid tissue.

  • Cellular localization

    Secreted

  • Alternative names

    • Alpha 1 type I collagen
    • Alpha 2 type I collagen
    • COL1A1
    • COL1A2

  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab216064 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA Use at an assay dependent concentration.

    Images

    • Human Pro-Collagen I alpha 1 standard curve.
      Human Pro-Collagen I alpha 1 standard curve.

      Standard calibration curve. Background subtracted values are graphed.

    Protocols

    References

    ab216064 has not yet been referenced specifically in any publications.

    Publishing research using ab216064? Please let us know so that we can cite the reference in this datasheet.

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