Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit (ab216064)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit
See all Procollagen Type 1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
8.44 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Pro-Collagen I alpha 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Pro-Collagen I alpha 1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210966. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Pro-Collagen I alpha 1 Capture Antibody 2 x 50µg 1 x 50µg Human Pro-Collagen I alpha 1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Pro-Collagen I alpha 1 Lyophilized Protein 1 vial 1 vial -
Research areas
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Relevance
Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Procollagen type 1 is mainly synthesized by osteoblasts and, after cleavage of the N- and C-terminal extension peptides, is utilized for collagen fibril deposition in the osteoid tissue. -
Cellular localization
Secreted -
Alternative names
- Alpha 1 type I collagen
- Alpha 2 type I collagen
- COL1A1
- COL1A2
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Database links
- Entrez Gene: 1278 Human
- Entrez Gene: 1277 Human
- SwissProt: P02452 Human
- SwissProt: P08123 Human
Associated products
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Alternative Versions
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Related Products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab216064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. |
Images
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Human Pro-Collagen I alpha 1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit (ab216064)To learn more about the advantages of recombinant antibodies see here.
References (0)
ab216064 has not yet been referenced specifically in any publications.
