Product nameHuman Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP)
See all PICP kits
Intra-assay Sample n Mean SD CV% Serum 8 3.1% Inter-assay Sample n Mean SD CV% Serum 3 7.2%
Sample typeSerum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range23.44 pg/ml - 1500 pg/ml
Sample specific recovery Sample type Average % Range Serum 102 92% - 109% Cell culture media 107 103% - 109% Hep Plasma 89 85% - 92% EDTA Plasma 89 85% - 100% Cit plasma 90 80% - 106%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human Pro-Collagen I C-Terminal Propeptide ELISA kit (PICP) (ab272198) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pro-Collagen I C-Terminal Propeptide protein in serum, plasma and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PICP with 8.15 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Pro-Collagen I C-Terminal Propeptide (PICP), encoded by the COL1A1 gene, is the fibrillar collagen that serves as a major component of type I collagen found in cartilage and most connective tissues. Studies have shown that mutations in the mutations in the Pro-Collagen I C-Terminal Propeptide are associated with osteoporosis, osteogenesis imperfecta diseases, and Ehlers-Danlos syndrome. The standard protein in this product is the C-terminal Propeptide region of Collagen alpha-1(I).
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human Pro-Collagen I C-Terminal Propeptide (PICP) Capture Antibody 1 x 600µl 10X Human Pro-Collagen I C-Terminal Propeptide (PICP) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 1ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Pro-Collagen I C-Terminal Propeptide (PICP) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Involvement in diseaseCaffey disease
Ehlers-Danlos syndrome 1
Ehlers-Danlos syndrome 7A
Osteogenesis imperfecta 1
Osteogenesis imperfecta 2
Osteogenesis imperfecta 3
Osteogenesis imperfecta 4
A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
Sequence similaritiesBelongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain.
DomainThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Alpha 1 type I collagen
- Alpha-1 type I collagen
ab272198 has not yet been referenced specifically in any publications.