Overview

  • Product name

    Human proBNP ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.14 ng/ml
  • Range

    0.14 ng/ml - 100 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 139.7 126% - 147%
    Plasma 126.3 103% - 150%
    Cell culture media 129.9 110% - 150%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s proBNP Human ELISA Kit (ab193712) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human proBNP in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human proBNP coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures proBNP present in the samples. The wells are washed and biotinylated anti-Human proBNP antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of proBNP bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated Human proBNP detection antibody (lyophilized) 2 vials
    Human proBNP Standards (lyophilized) 2 vials
    Pre-coated Human proBNP Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function

    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • Tissue specificity

    Brain and also in atria, but at much lower levels than ANP.
  • Sequence similarities

    Belongs to the natriuretic peptide family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • ANFB_HUMAN
    • BNP(1-32)
    • BNP(5-29)
    • BNP-32
    • Gamma-brain natriuretic peptide
    • nppb
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab193712.

Protocols

References

ab193712 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab193712.
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