• Product name
    Human Procalcitonin ELISA Kit
  • Detection method
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    27.43 pg/ml - 20000 pg/ml
  • Recovery

    83 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100.5 80% - 111%
    Plasma 72.35 65% - 88%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Procalcitonin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Procalcitonin in plasma and cell culture supernatants. (Human Procalcitonin concentration is pretty low in normal plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human Procalcitonin coated on a 96-well plate. Standards and samples are pipetted into the wells and Procalcitonin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Procalcitonin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Procalcitonin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect endogenous Human Procalcitonin in normal serum with ab100630, only in serum spiked with Human Procalcitonin.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab100630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab100630.

  • Representative Standard Curve using ab100630.



This product has been referenced in:
  • Ingram JR  et al. Levels of wound calprotectin and other inflammatory biomarkers aid in deciding which patients with a diabetic foot ulcer need antibiotic therapy (INDUCE study). Diabet Med 35:255-261 (2018). Read more (PubMed: 28734103) »
  • Wan B  et al. Rhubarb vs. glycerin enema for treatment of critically ill patients with intra-abdominal hypertension. Exp Ther Med 14:855-861 (2017). Read more (PubMed: 28673010) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Please recommend the customer to mix few micro litres of TMB solution with HRP-streptavidin solution on a glass slide. The colour developed should be blue which will change to yellow when stop solution is added. Read More

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1A, For storage please refer to the protocol and store the individual as given in the booklet.

"ab100630 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombina...

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The 8 well strips provided with ab100630 are not designed for single wells to be broken off and used individually.

Thank you for contacting us.

I am sorry for the delay in getting back to you. We are not open on the weekends. I am now able to confirm that you should be able to isolate individual wells of this product. As discussed in my previous email, the...

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Thank you for contacting Abcam. My colleague xxxxx passed on your enquiry to the Scientific Support
team and I hope I can help you with your questions.

The microplate provided with the Procalcitonin Human ELISA Kit (ab100630) is composed o...

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Thank you for contacting Abcam regarding ab100630.

Attached please find the QC of the standard for our most recent lot of kits. Regarding sensitivity, the minimum detectable dose of procalcitonin is typically less than 30 pg/ml.


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Thank you for contacting us.

To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the de...

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Thank you for contacting us.

The Procalcitonin Human ELISA Kit (ab100630) has to our knowledge not been compared to performing the Brahms method of detecting human procalcitonin. The kit should however match up well with most of the reported...

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I understand your reluctance to purchase a kit that might not detect macaque PCT. Unfortunately, we do not have any free kits, expired or otherwise.

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