Overview

  • Product name

    Human Prolactin ELISA Kit
    See all Prolactin kits
  • Detection method

    Colorimetric
  • Sample type

    Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 2 ng/ml
  • Range

    2 ng/ml - 200 ng/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Prolactin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prolactin in Human serum.


    A 96-well plate has been precoated with anti-Prolactin IgG antibodies. Samples and standards and are added to the wells, where Prolactin in the sample and standards binds to the precoated antibody. Added Anti-Prolactin-HRP conjugate binds to the antibody-Prolactin complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Prolactin in the sample and the intensity is measured at 450 nm.


    Get results in 90 minutes with Human Prolactin ELISA Kit (ab189570) from our SimpleStep ELISA® range.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Anti-Prolactin Coated Microplate (12 x 8 wells) 1 unit
    Anti-Prolactin HRP Conjugate 1 x 13ml
    Prolactin Standard 0 - 0 ng/mL (Lyophilized) 1 vial
    Prolactin Standard 1 - 5 ng/mL (Lyophilized) 1 vial
    Prolactin Standard 2 - 15 ng/mL (Lyophilized) 1 vial
    Prolactin Standard 3 - 50 ng/mL (Lyophilized) 1 vial
    Prolactin Standard 4 - 100 ng/mL (Lyophilized) 1 vial
    Prolactin Standard 5 - 200 ng/mL (Lyophilized) 1 vial
    Stop Solution 1 x 11ml
    TMB Reagent 1 x 11ml
  • Research areas

  • Relevance

    Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation.
  • Cellular localization

    Secreted
  • Alternative names

    • AV290867
    • Lactogenic Hormone
    • Luteotropic Hormone
    • Mammotropin
    • OTTMUSP00000000651
    • PRL
    • Prl1a1
    • Prol
    • Prolactin precursor
    • RATPRLSD1
    • RNPROL
    • RP23-351I6.2
    • RP3-404K8.1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab108655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab108655

Protocols

References

This product has been referenced in:

  • Zheng R  et al. A novel PNPLA6 compound heterozygous mutation identified in a Chinese patient with Boucher-Neuhäuser syndrome. Mol Med Rep 18:261-267 (2018). Read more (PubMed: 29749493) »
  • Kotb El-Sayed MI  et al. Neural and Endocrinal Pathobiochemistry of Vitiligo: Comparative Study for a Hypothesized Mechanism. Front Endocrinol (Lausanne) 9:197 (2018). Read more (PubMed: 29922226) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Serum samples to be used within 24-48 hours may be stored at 2-8 C.

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Answer

Thank you for contacting us. The Prolactin Human ELISA Kit (ab108655) is intended for the quantitative determination of prolactin concentration in human serum. We have not tested it with cell culture media and cannot say for certain if it will work. What type of cells are you culturing (type and species) and what other reagents would be present in the media (e.g. fetal calf serum)? Please let me know. I look forward to hear back from you.

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Answer

Gracias por ponerse en contacto con nosotros. Puede encontrar la información acerca de las condiciones de almacenamiento y caducidad de nuestros productos en las fichas técnicas de los mismos, que encontrará en nuestra web site www.abcam.com. En el caso del producto que nos refiere, y atendiendo a su ficha técnica: https://www.abcam.com/prolactin-human-elisa-kit-ab108655.html éste puede utilizarse durante 6 meses desde su recibimiento, siempre que se almacene a 4C. No olvide que nuestra garantía Abpromise es válida durante los siguientes 6 meses a la fecha de compra del producto, siempre y cuando se respeten las condiciones de almacenamiento requeridas. Espero que esta información le sea de utilidad. Por favor, para cualquier otra duda, información o sugerencia, no dude en ponerse en contacto con nosotros.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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