Key features and details
- Sensitivity: 0.25 ng/ml
- Range: 5 ng/ml - 100 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Prolactin ELISA Kit
See all Prolactin kits
Intra-assay Sample n Mean SD CV% Level 1 20 6.32 0.4 6.3% Level 2 20 19.22 1.19 6.18% Level 3 20 54.52 2.64 4.84% Inter-assay Sample n Mean SD CV% Level 1 20 6.58 0.45 6.77% Level 2 20 21.34 1.3 6.11% Level 3 20 58.39 2.93 5.01%
Assay typeSandwich (quantitative)
Range5 ng/ml - 100 ng/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Prolactin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prolactin in Human serum.
Microtiterstrip wells are precoated with inactivated specific Corynebacterium diphtheriae toxin (toxoid) antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured C. diphtheriae toxin-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of C. diphtheriae toxin-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
Get results in 90 minutes with Human Prolactin ELISA Kit (ab189570) from our SimpleStep ELISA® range.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 10X Wash Solution 1 x 50ml Anti-Prolactin-HRP conjugate 1 x 12ml Cover foil 1 unit Prolactin control 1 x 1ml Prolactin Standard 0: 0 ng/ml 1 x 1ml Prolactin Standard 1: 5 ng/ml 1 x 1ml Prolactin Standard 2: 10 ng/ml 1 x 1ml Prolactin Standard 3: 25 ng/ml 1 x 1ml Prolactin Standard 4: 50 ng/ml 1 x 1ml Prolactin Standard 5: 100 ng/ml 1 x 1ml Stop Solution 1 x 15ml Streptavidin Coated Microplate (12 x 8 wells) 12 Strips of 8 wells 1 unit Strip holder 1 unit TMB Substrate Solution 1 x 15ml
RelevanceVarious hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation.
- Lactogenic Hormone
- Luteotropic Hormone
Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
ab108679 has not yet been referenced specifically in any publications.