Overview

  • Product name

    Human Prorenin ELISA Kit
    See all Prorenin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 20 0.345ng/ml 0.02 5.74%
    2 20 0.464ng/ml 0.017 3.61%
    3 20 6.22ng/ml 0.237 3.81%
    Inter-assay
    Sample n Mean SD CV%
    1 10 0.342ng/ml 0.045 13.2%
    2 10 0.439ng/ml 0.056 12.6%
    3 10 6.16ng/ml 0.353 5.72%
  • Sample type

    Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.013 ng/ml
  • Range

    0.02 ng/ml - 10 ng/ml
  • Recovery

    99.75 %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Prorenin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for is for the quantitative determination of Prorenin in Human plasma. Active renin will not be detected by this assay. Prorenin is measured directly by ELISA without pre-treatment of samples or conversion to renin. 

    Human Prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-Human Prorenin primary antibody binds to the captured protein. Only Prorenin and not active renin will be detected by the primary antibody. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Prorenin. The amount of color development is directly proportional to the concentration of Prorenin in the sample.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 50ml
    Anti-Human Prorenin antibody coated plates 1 unit
    Anti-Human Prorenin Primary Antibody 1 vial
    Anti-mouse HRP Secondary Antibody 1 vial
    Human Prorenin Negative Control (0 ng) 2 vials
    Human Prorenin Positive Control (20 ng) 1 vial
    TMB substrate solution 1 x 10ml
  • Research areas

  • Function

    Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
  • Involvement in disease

    Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
    Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
  • Sequence similarities

    Belongs to the peptidase A1 family.
  • Cellular localization

    Secreted. Membrane. Associated to membranes via binding to ATP6AP2.
  • Information by UniProt
  • Alternative names

    • Angiotensinogenase
    • REN
    • RENI_HUMAN
    • Renin
  • Database links

Applications

Our Abpromise guarantee covers the use of ab157525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab157525 has not yet been referenced specifically in any publications.

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