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|Sample type||Average %||Range|
|Cell culture media||108.75||98% - 132%|
Abcam’s Prorenin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of Prorenin in non-plasma samples including cell culture media, tissue extracts, or urine samples. Active renin will not be detected by this assay. Prorenin is measured directly by ELISA without pretreatment of samples or conversion to renin.
Human Prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-Human Prorenin primary antibody binds to the captured protein. Only Prorenin and not active renin will be detected by the primary antibody. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Prorenin. The amount of color development is directly proportional to the concentration of Prorenin in the sample.
|Components||1 x 96 tests|
|10X Wash Buffer||1 x 50ml|
|Anti-Human Prorenin antibody coated plates||1 unit|
|Anti-Human Prorenin Primary Antibody||1 vial|
|Anti-mouse HRP Secondary Antibody||1 vial|
|Human Prorenin Standard||1 vial|
|TMB substrate solution||1 x 10ml|
Our Abpromise guarantee covers the use of ab169738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab169738 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"