Overview

  • Product name

    Human Prostasin ELISA Kit
    See all PRSS8 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 16ng/ml 0.67 4.2%
    2 16 42ng/ml 1.51 3.6%
    3 16 71ng/ml 2.71 3.8%
    Inter-assay
    Sample n Mean SD CV%
    1 24 20ng/ml 1.46 7.3%
    2 24 46ng/ml 2.62 5.7%
    3 24 75ng/ml 3.68 4.9%
  • Sample type

    Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 50 pg/ml
  • Range

    1.56 ng/ml - 10 ng/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Prostasin Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213817) is designed for the quantitative measurement of Human Prostasin in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from sheep specific for Prostasin has been pre-coated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: A33-G319) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for Prostasin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Prostasin amount of sample captured in plate.

  • Notes

    Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Co-expression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human Prostasin coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human Prostasin antibody 1 x 130µl
    Lyophilized recombinant Human Prostasin standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Possesses a trypsin-like cleavage specificity.
  • Tissue specificity

    Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Cell membrane and Secreted > extracellular space. Found in the seminal fluid. Secreted after cleavage of its C-terminus.
  • Information by UniProt
  • Alternative names

    • 2410039E18Rik
    • AI313909
    • C79772
    • CAP1
    • Channel Activating Protease 1
    • mCAP1
    • Prostasin
    • Prostasin heavy chain
    • protease, serine, 8
    • Prss8
    • PRSS8_HUMAN
    • Serine protease 8
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Prostasin ELISA Kit (ab213817) Standard Curve.

Protocols

References

ab213817 has not yet been referenced specifically in any publications.

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