Key features and details
- Sensitivity: 66 ng/ml
- Range: 0.19 µg/ml - 1.5 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Product nameHuman Protein C ELISA Kit
See all Protein C kits
Intra-assay Sample n Mean SD CV% Intra-assay 5.2% Inter-assay Sample n Mean SD CV% Inter-assay 10.1%
Sample typeSerum, Plasma
Range0.19 µg/ml - 1.5 µg/ml
Assay time3h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Protein C Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Protein C in plasma and serum.
A Protein C specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Protein C is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Protein C captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 1X Biotinylated Human Protein C (Lyophilized) 1 vial 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 20X Wash Buffer Concentrate 1 x 30ml Chromogen Substrate 1 x 7ml Protein C Microplate (12 x 8 well strips) 1 unit Protein C Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml
FunctionProtein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
Tissue specificityPlasma; synthesized in the liver.
Involvement in diseaseThrombophilia due to protein C deficiency, autosomal dominant
Thrombophilia due to protein C deficiency, autosomal recessive
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
modificationsThe vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
Cellular localizationSecreted. Golgi apparatus. Endoplasmic reticulum.
- Information by UniProt
- Activation peptide
- Anticoagulant protein C
ab137988 has not yet been referenced specifically in any publications.