Overview

  • Product name

    Human Protein S ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.4%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    0.25 µg/ml
  • Range

    0.25 µg/ml - 8 µg/ml
  • Recovery

    98.5 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Protein S Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human protein S in plasma, serum and cell culture supernatants.

    A Protein S specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Protein S is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Protein S captured in plate.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 1 x 30ml
    1X Biotinylated Human Protein S (Lyophilized) 1 vial
    Chromogen Substrate 1 x 8ml
    Protein S Microplate (12 x 8 well strips) 1 unit
    Protein S Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in PROS1 are the cause of protein S deficiency (PROS1D) [MIM:612336]; also known as thrombophilia due to protein S deficiency. PROS1D is a cause of hereditary thrombophilia, a hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Based on the plasma levels of total and free PROS1 antigen as well as the serine protease-activated protein C cofactor activity, three types of PROS1D have been described: type I, characterized by reduced total and free PROS1 antigen levels together with reduced anticoagulant activity; type III, in which only free PROS1 antigen and PROS1 activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free PROS1 antigen, but low cofactor activity.
  • Sequence similarities

    Contains 4 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 laminin G-like domains.
  • Post-translational
    modifications

    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Preproprotein S
    • Propiece of latent protein S
    • PROS
    • PROS 1
    • PROS_HUMAN
    • proS1
    • Protein S alpha
    • Protein Sa
    • PS 21
    • PS 22
    • PS 23
    • PS 24
    • PS 25
    • PS 26
    • PS21
    • PS22
    • PS23
    • PS24
    • PS25
    • PS26
    • PSA
    • THPH5
    • THPH6
    • Vitamin K dependent protein S
    • Vitamin K-dependent plasma protein S
    • Vitamin K-dependent protein S
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab125969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab125969

Protocols

References

This product has been referenced in:

See 1 Publication for this product

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