Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.4 ng/ml
- Range: 3.12 ng/ml - 200 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Protein S ELISA Kit
Intra-assay Sample n Mean SD CV% EDTA Plasma 5 1.8% Inter-assay Sample n Mean SD CV% EDTA Plasma 3 2.5%
Sample typeSerum, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range3.12 ng/ml - 200 ng/ml
Sample specific recovery Sample type Average % Range Serum 88.7 86% - 93.4% Hep Plasma 85.7 78.6% - 91.1% EDTA Plasma 89.3 86.6% - 92.8% Cit plasma 90.4 87% - 94%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Does not react with: Goat, Cow, Pig
Human Protein S ELISA Kit is being re-developed with new recombinant monoclonal antibodies to provide improved performance and consistency. This version will be discontinued when inventory is depleted.
Human Protein S ELISA Kit (ab190808) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Protein S protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Protein S with 0.4 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Protein S (vitamin K-dependent protein S) is an anticoagulant plasma protein. Protein S is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis. Protein S deficiency causes autosomal dominant (THP5) [MIM: 612336] and autosomal recessive (THP6) [MIM: 614514] thrombophilia. The autosomal dominant thrombophilia due to Protein S deficiency (THPH5) is hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. Based on the plasma levels of total and free Protein S as well as the serine protease-activated protein C cofactor activity, three types of THPH5 have been described: type I, characterized by reduced total and free Protein S levels together with reduced anticoagulant activity; type III, in which only free Protein S antigen and Protein S activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free Protein S antigen, but low cofactor activity. The autosomal recessive thrombophilia due to Protein S deficiency (THPH6) is a very rare and severe hematologic disorder resulting in thrombosis and secondary hemorrhage usually beginning in early infancy. Some affected individuals develop neonatal purpura fulminans, multifocal thrombosis, or intracranial hemorrhage.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human Protein S Capture Antibody 1 x 600µl 10X Human Protein S Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Protein S Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
RelevanceFunction: Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.
- Protein S (alpha)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of Protein S were measured in duplicates, interpolated from the Protein S standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 3,200, plasma (citrate) 1: 3,200, plasma (EDTA) 1: 3,200, and plasma (heparin) 1: 1,600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Protein S concentration was determined to be 29.75 µg/mL in serum, 22.13 µg/mL in plasma (citrate), 25.10 µg/mL in plasma (EDTA). and 11.70 µg/mL in plasma (heparin).
200 X diluted serum samples from 10 apparently healthy male donors were measured in triplicates using this kit. Interpolated data values corrected for sample dilution are graphed in ng of Protein S per mL of serum (mean +/- SD, n=3). The mean of Protein S concentration of these serum samples was determined to be 22,623 pg/mL with a range of 19,138 – 28,150 ng/mL.
ab190808 has been referenced in 1 publication.
- Chang CY et al. Effects of Caloric Restriction with Protein Supplementation on Plasma Protein Profiles in Middle-Aged Women with Metabolic Syndrome-A Preliminary Open Study. J Clin Med 8:N/A (2019). PubMed: 30736312