Overview

  • Product name

    Human Protein S ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    EDTA Plasma 5 1.8%
    Inter-assay
    Sample n Mean SD CV%
    EDTA Plasma 3 2.5%
  • Sample type

    Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.4 ng/ml
  • Range

    3.12 ng/ml - 200 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 88.7 86% - 93.4%
    Heparin Plasma 85.7 78.6% - 91.1%
    EDTA Plasma 89.3 86.6% - 92.8%
    Citrate Plasma 90.4 87% - 94%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Pig
  • Product overview

    Protein S in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Protein S protein in human serum and plasma samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Protein S (vitamin K-dependent protein S) is an anticoagulant plasma protein. Protein S is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis. Protein S deficiency causes autosomal dominant (THP5) [MIM: 612336] and autosomal recessive (THP6) [MIM: 614514] thrombophilia. The autosomal dominant thrombophilia due to Protein S deficiency (THPH5) is hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. Based on the plasma levels of total and free Protein S as well as the serine protease-activated protein C cofactor activity, three types of THPH5 have been described: type I, characterized by reduced total and free Protein S levels together with reduced anticoagulant activity; type III, in which only free Protein S antigen and Protein S activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free Protein S antigen, but low cofactor activity. The autosomal recessive thrombophilia due to Protein S deficiency (THPH6) is a very rare and severe hematologic disorder resulting in thrombosis and secondary hemorrhage usually beginning in early infancy. Some affected individuals develop neonatal purpura fulminans, multifocal thrombosis, or intracranial hemorrhage.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Protein S Capture Antibody 1 x 600µl
    10X Human Protein S Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Plate Seals 1 unit
    Human Protein S Lyophilized Purified Protein 2 vials
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    Function: Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.
  • Alternative names

    • PROS
    • PROS1
    • Protein S (alpha)
    • Protein Sa
    • PS21
    • PS22
    • PS23
    • PS24
    • PS25
    • PSA
    • THPH5
    • THPH6
    • Vitamin K dependent plasma protein S
    • Vitamin K dependent protein S
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab190808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • 200 X diluted serum samples from 10 apparently healthy male donors were measured in triplicates using this kit. Interpolated data values corrected for sample dilution are graphed in ng of Protein S per mL of serum (mean +/- SD, n=3). The mean of Protein S concentration of these serum samples was determined to be 22,623 pg/mL with a range of 19,138 – 28,150 ng/mL.

Protocols

References

ab190808 has not yet been referenced specifically in any publications.

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