Overview

  • Product name

    Human Protein Z ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.7%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    > 0.9 ng/ml
  • Range

    89 % - 112 %
  • Recovery

    96 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Protein Z Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Protein Z in plasma, serum, urine, milk, and cell culture supernatants.

    A Protein Z specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Protein Z specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Protein Z captured in plate.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Protein Z Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Protein Z Microplate (12 x 8 well strips) 1 unit
    Protein Z Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles.
  • Tissue specificity

    Plasma.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • PROZ
    • PROZ_HUMAN
    • Vitamin K-dependent protein Z
  • Database links

Applications

Our Abpromise guarantee covers the use of ab137989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Protein Z measured in biological fluids (duplicates +/- SD). Human plasma and serum were tested at a dilution factor range of 50-1350, other fluids 1-10.

  • Representative Standard Curve using ab137989.

Protocols

References

ab137989 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137989.
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