• Product name
    Human PSA ELISA Kit (KLK3)
    See all Prostate Specific Antigen kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall < 10%
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    10.24 pg/ml - 2500 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.25 81% - 108%
    Serum 105.4 94% - 112%
    Plasma 80.63 72% - 94%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PSA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PSA in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human PSA coated on a 96-well plate. Standards and samples are pipetted into the wells and PSA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PSA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PSA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    Get results in 90 minutes with Human Total Prostate Specific Antigen ELISA Kit (ab188388) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


Associated products


Our Abpromise guarantee covers the use of ab113327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • PSA (KLK3) measured in various biological fluids showing quantity (pg) per mL of sample tested

  • Representative standard curve using ab113327

  • Representative standard curve using ab113327



This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

Assay diluent A is is only required for the preparation of the standards and samples, if your samples are plasma. Once the PSA is captured on the plate and the samples and standard solutions are discarded from the wells, the remainder of the assay does...

Read More

Thank you for contacting us. Your credit note ID is . I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing de...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

Read More

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments, and would like to off...

Read More

Thank you for your response.

I am sorry that the initial suggestion that I was able to give and that these further suggestions did not improve blank reading and thereby the detection limits of this for you. Would you be willing to send me th...

Read More

Thank you for contacting Abcam regarding your higher 0 reading.

I have had a chance to speak with the lab regarding this. Their reply follows:

In general, a 0 standard OD reading around 0.2 OD is not considered very high but is in...

Read More

Code de remise : XX
Date d’expiration : 09. Juillet 2012

Je suis ravie que vous souhaitiez participer a notre offre et tester ab113327 chez le macaque. Le code de réduction correspondant est XX. Ce code de réduction est utilisable sous ...

Read More

Thank you for your inquiry. Although our production team does not’t routinely test urine samples,we know this kit is suitable for urine samples (based on our past experience with it). This is a general guideline on preparation of urine for ELIS...

Read More


Sign up