Overview

  • Product name
    Human PTP lambda peptide

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab13230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-PTP lambda antibody (ab13223)

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Pancreatic carcinoma phosphatase 2
    • PCP-2
    • Protein-tyrosine phosphatase J
    • PTP-J
    • PTPRU
    • R-PTP-U
    • Receptor-type tyrosine-protein phosphatase U
    see all
  • Relevance
    PTP lambda is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle and oncogenic transformation. PTP lambda is a large protein that possesses a C-terminus PTP domain and multiple noncatalytic domains which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains and a leucine zipper motif. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. PTP lambda is up-regulated upon cell contact and is found in high levels in brain, pancreas, and skeletal muscle; less in colon, kidney, liver, stomach and uterus. It is not expressed in placenta and spleen.
  • Cellular localization
    Type 1 membrane protein

Images

  • Wester Blot with ab13223 Rabbit polyclonal to PTP lambda (1/500)
    Secondary: Goat anti-rabbit IgG ab6721 (1/5000)
    Exposure time: 5 mins
    Expected molecular weight: 160 kDa

    Lanes 1 to 8: 20µg of cell lysate per lane
    Lanes 5 to 8: blocking peptide used at 1µg/ml

    Lane 1: Human Brain Lysate
    Lane 2: Human Kidney Lysate
    Lane 3: Human Pancreas Lysate
    Lane 4: Human Heart Lysate
    Lane 5: Human Brain Lysate + blocking peptide (ab13230)
    Lane 6: Human Kidney Lysate + blocking peptide (ab13230)
    Lane 7: Human Pancreas Lysate + blocking peptide (ab13230)
    Lane 8: Human Heart Lysate + blocking peptide (ab13230)

    A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230).

References

ab13230 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab13230.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up