Overview

  • Product name

    Human PYK2 Antibody Pair - BSA and Azide free
    See all PYK2 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    78 pg/ml - 5000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human PYK2. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab226892), which uses the same antibodies. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    To receive an electronic copy of the Certificate of Analysis, please send an email to technical support with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Download SDS here.


     

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 10 x 96 tests
    Human PYK2 Capture Antibody 1 x 100µg
    Human PYK2 Detector Antibody 1 x 100µg
  • Research areas

  • Function

    Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
  • Tissue specificity

    Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
  • Cellular localization

    Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
  • Information by UniProt
  • Alternative names

    • CADTK
    • CAK-beta
    • CAKB
    • CAKbeta
    • Calcium regulated non receptor proline rich tyrosine kinase
    • Calcium-dependent tyrosine kinase
    • Cell adhesion kinase beta
    • E430023O05Rik
    • EC 2.7.10.2
    • FADK 2
    • FADK2
    • FAK2
    • FAK2_HUMAN
    • Focal adhesion kinase 2
    • MGC124628
    • PKB
    • Proline-rich tyrosine kinase 2
    • Protein kinase B
    • Protein Tyrosine Kinase 2 Beta
    • Protein-tyrosine kinase 2-beta
    • PTK
    • PTK2B
    • PTK2B protein tyrosine kinase 2 beta
    • PYK2
    • RAFTK
    • RAFTK2
    • Related adhesion focal tyrosine kinase
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab244031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

References

ab244031 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab244031.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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