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The oxidation of carbohydrates in mammals is regulated by the pyruvate dehydrogenase (PDH) complex, which is covalently regulated by four PDH kinases (PDK1-4) and two PDH phosphatases (PDP1-2) through a phosphorylation-dephosphorylation cycle. PDK4 Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. PDK4 exists ubiquitous with highest levels of expression in heart and skeletal muscle. PDK4 transcription and PDK4 mRNA are markedly increased in human skeletal muscle during prolonged exercise and after both short-term high-intensity and prolonged low-intensity exercise. The exercise-induced transcriptional response of PDK4 is enhanced when muscle glycogen is lowered before the exercise, and intake of a low-carbohydrate high-fat diet during recovery from exercise results in increased transcription and mRNA content of PDK4 when compared with intake of a high-carbohydrate diet. PDK4 expression is also increased in response to fasting and a high-fat diet.
ab126582 PDK4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the accurate quantitative measurement of PDK4 protein in human tissue lysates. The assay employs an antibody specific to PDK4 protein coated onto well plate strips. Standards and samples are pipetted into the wells and PDK4 present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a primary anti-PDK4 detector antibody is added. After washing away unbound detector antibody, HRP-conjugated secondary antibody (HRP Label) specific for the primary detector antibody is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound PDK4. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
|Components||1 x 96 tests|
|10X Blocking Buffer||1 x 6ml|
|10X HRP Label||1 x 1ml|
|10X PDK4 Detector Antibody||1 x 1ml|
|20X Buffer||1 x 20ml|
|2X Extraction Buffer||1 x 10ml|
|PDK4 Human Standard (250 ng)||1 vial|
|PDK4 Microplate||1 unit|
|HRP Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab126582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab126582 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"