• Product name

    Human Pyruvate Dehydrogenase Kinase isoform 4 ELISA Kit
  • Detection method

  • Precision

    Sample n Mean SD CV%
    PDK4 3 4%
    Heart Lysate 3 6%
    Sample n Mean SD CV%
    PDK4 3 0.7%
    Heart Lysate 3 1.5%
  • Sample type

    Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 3.9 ng/ml
  • Range

    3.9 ng/ml - 250 ng/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The oxidation of carbohydrates in mammals is regulated by the pyruvate dehydrogenase (PDH) complex, which is covalently regulated by four PDH kinases (PDK1-4) and two PDH phosphatases (PDP1-2) through a phosphorylation-dephosphorylation cycle. PDK4 Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. PDK4 exists ubiquitous with highest levels of expression in heart and skeletal muscle. PDK4 transcription and PDK4 mRNA are markedly increased in human skeletal muscle during prolonged exercise and after both short-term high-intensity and prolonged low-intensity exercise. The exercise-induced transcriptional response of PDK4 is enhanced when muscle glycogen is lowered before the exercise, and intake of a low-carbohydrate high-fat diet during recovery from exercise results in increased transcription and mRNA content of PDK4 when compared with intake of a high-carbohydrate diet. PDK4 expression is also increased in response to fasting and a high-fat diet.

    ab126582 PDK4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the accurate quantitative measurement of PDK4 protein in human tissue lysates. The assay employs an antibody specific to PDK4 protein coated onto well plate strips. Standards and samples are pipetted into the wells and PDK4 present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a primary anti-PDK4 detector antibody is added. After washing away unbound detector antibody, HRP-conjugated secondary antibody (HRP Label) specific for the primary detector antibody is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound PDK4. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform



Associated products


Our Abpromise guarantee covers the use of ab126582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.


  • All lanes : Human Pyruvate Dehydrogenase Kinase isoform 4 ELISA Kit (ab126582)

    Lane 1 : HepG2 whole cell lysate (ab7900) at 20 µg
    Lane 2 : Human heart tissue lysate - total protein (ab29431) at 20 µg
    Lane 3 : PDK1 protein at 0.1 µg
    Lane 4 : PDK2 protein at 0.1 µg
    Lane 5 : PDK3 protein at 0.1 µg
    Lane 6 : PDK4 protein at 0.1 µg

    Antibody Specificity was determined by western blotting Human PDK, PDK2, PDK3 and PDK4 recombinant proteins. Capture antibody reacts with Human samples and specific to PDK4 protein.
  • Assay Specificity was determined by using human PDK1 recombinant protein as negative control.
  • PDK4 exists ubiquitous in tissues with highest levels of expression in heart (HHH) and skeletal muscle (HSMH). This ELISA assay applies specifically to human samples. No signal was observed when using rat tissue (rat heart homogenate (RHH)) or mouse tissue (mouse heart homogenate (MHH)).
  • Interference by cell culture media (20F DMEM), Goat serum, and a titration of 1X extraction buffer were evaluated.
  • Example human sample curve of serially diluted human heart homogenate extract in the working range of the assay.
  • Example standard curve of serially diluted purified human PDK4 in the linear working range of the assay.



ab126582 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab126582.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up