Human QPRT ELISA Kit (ab284020)
Key features and details
- Sensitivity: 0.33 ng/ml
- Range: 0.781 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human QPRT ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.5% Inter-assay Sample n Mean SD CV% Overall 10.1% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
0.33 ng/ml -
Range
0.781 ng/ml - 25 ng/ml -
Recovery
98 %
Sample specific recovery Sample type Average % Range Plasma 98 91% - 112% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Quinolinate phosphoribosyltransferase (QPRT) belongs to the phosphoribosyltransferase family and is involved in de novo NAD biosynthesis using quinolinate as a precursor. Quinolinate acts as a most potent endogenous exitotoxin to neurons, causing neuronal damage through sustained activation of glutamate N-methyl-D-aspartate receptors.
Human QPRT ELISA (Enzyme-Linked Immunosorbent Assay) Kit is designed for detection of QPRT in human plasma, serum, milk, urine, saliva, CSF, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human QPRT in approximately 4 hours. A polyclonal antibody specific for human QPRT has been pre-coated onto a 96-well microplate with removable strips. QPRT in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human QPRT, which is recognized by a streptavidin-peroxidase (SP) conjugate. The color development is stopped and the intensity of the color is measured.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Human QPRT Antibody 1 vial Chromogen Substrate 1 vial Human QPRT Microplate 1 unit QPRT Standard 1 vial Sealing Tapes 3 units Stop Solution 1 vial -
Research areas
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Function
Involved in the catabolism of quinolinic acid (QA). -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1. -
Sequence similarities
Belongs to the nadC/modD family. - Information by UniProt
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Alternative names
- NADC_HUMAN
- Nicotinate nucleotide pyrophosphorylase (carboxylating)
- Nicotinate-nucleotide pyrophosphorylase [carboxylating]
see all -
Database links
- Entrez Gene: 23475 Human
- Omim: 606248 Human
- SwissProt: Q15274 Human
- Unigene: 513484 Human
Images
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Sandwich ELISA - Human QPRT ELISA Kit (ab284020)Example of Human QPRT standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284020 has not yet been referenced specifically in any publications.