Human Receptor Antibody Array (Membrane, 40 Targets) (ab211065)

Overview

  • Product name

    Human Receptor Antibody Array (Membrane, 40 Targets)
  • Sample type

    Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam's Human Receptor Antibody Array (ab211065) for use with serum, plasma, cell culture media, cell lysate, tissue lysate and other liquid sample types.


    Targets: 4-1BB (TNFRSF9), ALCAM (CD166), CD80 (B7-1), BCMA (TNFRSF17), CD14, CD30 (TNFRSF8), CD40 Ligand (TNFSF5), CEACAM-1, DR6 (TNFRSF21), Dtk, Endoglin (CD105), ErbB3, E-Selectin, Fas (TNFRSF6), Flt-3 Ligand, GITR (TNFRSF18), HVEM (TNFRSF14), ICAM-3 (CD50), IL-1 R4 (ST2), IL-1 R1, IL10 Rbeta, IL-17RA, IL-1 R gamma, IL-21R, LIMPII, Lipocalin-2 (NGAL), L-Selectin (CD62L), LYVE-1, MICA, MICB, NRG1-beta 1, PDGF R beta, PECAM-1 (CD31), RAGE, TIM-1 (KIM-1), TRAIL R3 (TNFRSF10C), Trappin-2, uPar, VCAM-1 (CD106), XEDAR.

  • Notes

    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

    Learn more about cytokine arrays and other membrane antibody arrays

  • Tested applications

    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 2 Membrane 4 Membrane 8 Membrane
    1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl
    20X Wash Buffer I 1 x 10ml 1 x 10ml 1 x 20ml
    20X Wash Buffer II 1 x 10ml 1 x 10ml 1 x 20ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Antibody Arrays 2 units 4 units 8 units
    Biotinylated Antibody Cocktail 2 vials 2 vials 4 vials
    1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml
    Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml
    Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml
    Plastic sheets 1 unit 1 unit 1 unit

Applications

Our Abpromise guarantee covers the use of ab211065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

Protocols

References

ab211065 has not yet been referenced specifically in any publications.

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