Product nameHuman Receptor Antibody Array (Membrane, 40 Targets)
Sample typeSerum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
Species reactivityReacts with: Human
Abcam's Human Receptor Antibody Array (ab211065) for use with serum, plasma, cell culture media, cell lysate, tissue lysate and other liquid sample types.
Targets: 4-1BB (TNFRSF9), ALCAM (CD166), CD80 (B7-1), BCMA (TNFRSF17), CD14, CD30 (TNFRSF8), CD40 Ligand (TNFSF5), CEACAM-1, DR6 (TNFRSF21), Dtk, Endoglin (CD105), ErbB3, E-Selectin, Fas (TNFRSF6), Flt-3 Ligand, GITR (TNFRSF18), HVEM (TNFRSF14), ICAM-3 (CD50), IL-1 R4 (ST2), IL-1 R1, IL10 Rbeta, IL-17RA, IL-1 R gamma, IL-21R, LIMPII, Lipocalin-2 (NGAL), L-Selectin (CD62L), LYVE-1, MICA, MICB, NRG1-beta 1, PDGF R beta, PECAM-1 (CD31), RAGE, TIM-1 (KIM-1), TRAIL R3 (TNFRSF10C), Trappin-2, uPar, VCAM-1 (CD106), XEDAR.
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Tested applicationsSuitable for: Multiplex Protein Detectionmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 2 Membrane 4 Membrane 8 Membrane 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Antibody Arrays 2 units 4 units 8 units Biotinylated Antibody Cocktail 2 vials 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Plastic sheets 1 unit 1 unit 1 unit
Our Abpromise guarantee covers the use of ab211065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
ab211065 has not yet been referenced specifically in any publications.