Overview

  • Product name

    Human Resistin ELISA Kit (RETN)
    See all Resistin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7%
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.2 ng/ml
  • Range

    0.25 ng/ml - 16 ng/ml
  • Recovery

    = 97 %

  • Assay time

    5h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Resistin (RETN) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Resistin concentrations in Human plasma, serum, urine, saliva and cell culture supernatants.


    A Resistin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Resistin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Resistin captured in plate.


    Get results in 90 minutes with Human Resistin ELISA Kit (ab183364) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108896.

Protocols

References

This product has been referenced in:

  • Al-Rawi N & Al-Marzooq F The Relation between Periodontopathogenic Bacterial Levels and Resistin in the Saliva of Obese Type 2 Diabetic Patients. J Diabetes Res 2017:2643079 (2017). Read more (PubMed: 29138754) »
See 1 Publication for this product

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