Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Respiratory syncytial virus IgA ELISA Kit (RSV)
Intra-assay Sample n Mean SD CV% Pos.Serum 24 4.9% Inter-assay Sample n Mean SD CV% Pos.Serum 12 5.2%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Respiratory syncytial virus (RSV) IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Respiratory syncytial virus in Human serum and plasma.
A 96-well plate has been precoated with Respiratory syncytial virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Respiratory syncytial virus -specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Respiratory syncytial virus IgA sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Anti-Respiratory syncytial virus (IgA) Coated Microplate (12 x 8 wells) 1 unit Foil cover 1 unit IgA Sample Diluent yellow buffer, white cap 1 x 100ml Respiratory syncytial virus anti-IgA HRP Conjugate violet solution, black cap 1 x 20ml Respiratory syncytial virus IgA Cut-off Control yellow solution, green cap 1 x 3ml Respiratory syncytial virus IgA Negative Control yellow solution, blue cap 1 x 2ml Respiratory syncytial virus IgA Positive Control yellow solution, red cap 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
- RNA Virus
- single stranded RNA negative strand virus
- Resp. Syncytial Virus
ab108764 has not yet been referenced specifically in any publications.